Overexpression of CAV3 facilitates bone formation via the Wnt signaling pathway in osteoporotic rats
- PDF / 2,357,396 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 1 Downloads / 181 Views
ORIGINAL ARTICLE
Overexpression of CAV3 facilitates bone formation via the Wnt signaling pathway in osteoporotic rats Run-Bao Yang1 Feng-Fei Lin2 Jun Yang1 Bin Chen2 Ming-Hua Zhang1 Qiao-Ping Lu1 Bo Xiao1 Yan Liu1 Ke Zheng2 Yong-Rong Qiu3 ●
●
●
●
●
●
●
●
●
1234567890();,:
1234567890();,:
Received: 6 June 2018 / Accepted: 20 October 2018 / Published online: 14 November 2018 © Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract Purpose Osteoporosis is a condition characterized by decreased bone density and bone strength, commonly observed among older individuals. Caveolin-3 (CAV3) is a principal structural protein of the caveolae membrane domains, which has been reported to participate in cell signaling as well as the maintenance of cell structure. The aim of the current study was to investigate the effects involved with the silencing of CAV3 on bone formation among osteoporotic rat models via the Wnt signaling pathway. Methods Osteoporosis was initially induced by means of ovariotomy among rat models in order to determine the expression of CAV3. Then, to confirm the specific function and mechanism of CAV3 from an osteoporosis perspective, the CAV3 expression vector was constructed and transfected into the osteoblasts of the osteoporotic rats. Afterward, the mRNA and protein expressions of CAV3, β-catenin, low-density lipoprotein receptor-related protein 5 (LRP5), T-cell factor (TCF), and Wnt3a in addition to cell proliferation and apoptosis were detected accordingly. Results Positive expression of CAV3 exhibited diminished levels in the bone tissues of osteoporotic rats. The osteoblasts of the osteoporotic rats treated with overexpressed CAV3 displayed elevated mRNA and protein expression levels of β-catenin, LRP5, TCF, and Wnt3a. Increased cell proliferation and decreased cell apoptosis were also observed, while the osteoblasts of the osteoporotic rats treated with si-CAV3 exhibited an opposite result. Conclusion Overexpressed CAV3 promotes bone formation and suppresses the osteoporosis progression via the activation of the Wnt signaling in rat models, suggesting CAV3 as a potential target biomarker in the treatment of osteoporosis. Keywords Osteoporosis CAV3 Gene silencing Wnt signaling pathway Bone formation ●
●
●
Introduction Osteoporosis is widely considered to be a significant public health stumbling block, often increasing an individual’s
These authors are regarded as co-first authors: Run-Bao Yang, FengFei Lin. * Yong-Rong Qiu [email protected] 1
Department of Orthopedics and Traumatology, Longyan First Hospital, Longyan 364000, P. R. China
2
Department of Orthopedic Surgery, Fuzhou Second Hospital, Fuzhou 350007, P. R. China
3
Department of Orthopaedics Surgery, Longyan First Hospital, Longyan 364000, P. R. China
●
susceptibility to fracture risk, particularly among the aged population, with the hip, humerus, and vertebrae particularly at risk [1]. Osteoporosis represents a disease resulting in decreased bone density and diminishing bone strength affecting bone sup
Data Loading...
