Overexpression of miR1861h increases tolerance to salt stress in rice ( Oryza sativa L.)
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SHORT COMMUNICATION
Overexpression of miR1861h increases tolerance to salt stress in rice (Oryza sativa L.) Bin Ai . Yong Chen . Minmin Zhao . Gumu Ding . Jiankun Xie . Fantao Zhang
Received: 31 March 2020 / Accepted: 10 October 2020 Ó Springer Nature B.V. 2020
Abstract Salt stress is one of the most devastating abiotic stresses for global rice production. The conserved miR1861 family has been shown to participate in the control of plant growth, development, and response to abiotic stresses. However, no reports have focused yet on the roles of miR1861 in the salt stress responses. In a previous study, the member miR1861h was identified in Dongxiang wild rice (DXWR, Oryza rufipogon Griff.) with small RNA sequencing. In this study, we found that the expression level of miR1861h increased significantly under salt stress. Then, we generated transgenic plants overexpressing miR1861h under control of the CaMV35S promoter, and verified miR1861h expression using qRT-PCR analysis. We found that miR1861h overexpression increased plant salt stress tolerance significantly, as evidenced by higher survival rates. Analysis of putative targets and cis-acting elements further suggested that miR1861h
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-01045-9) contains supplementary material, which is available to authorized users. B. Ai Y. Chen M. Zhao G. Ding J. Xie (&) F. Zhang (&) Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China e-mail: [email protected] F. Zhang e-mail: [email protected]
could be a positive regulator of plant tolerance to salt stress. Taken together, our findings provide more insight into the roles of miR1861 family. Keywords Genetic resource MiRNA Regulator Rice Salt stress tolerance
Introduction Rice (Oryza sativa L.), a staple food for more than half of the world’s population, is a typical glycophyte, and is considered to be one of the most salt-sensitive cereal crops (Patishtan et al. 2018). It is estimated that saltaffected soils currently account for nearly 20% of global rice planting areas (Li et al. 2018). In recent years, numerous studies have shown that salt stress can lead to serious adverse effects in rice plant height, panicle length, seed setting rate, grain composition, and yield (Ganie et al. 2019). Salt stress has become one of the most severe environmental threats to global rice production (Almeida et al. 2017). Therefore, unraveling the underlying mechanisms associated with salt stress tolerance has been the focus of increasing attention, with the ultimate goal of improving the salt stress tolerance of rice. MicroRNAs (miRNAs) are a type of small (20–24 nucleotides) noncoding RNA molecules that can negatively regulate the expression of target genes (Bartel 2009). In recent years, the function of miRNAs
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Genet Resour Crop Evol
as a vital regulator
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