Overexpression of SlMYB75 enhances resistance to Botrytis cinerea and prolongs fruit storage life in tomato
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ORIGINAL ARTICLE
Overexpression of SlMYB75 enhances resistance to Botrytis cinerea and prolongs fruit storage life in tomato Mengyu Liu1 · Zhen Zhang1 · Zhixuan Xu1 · Lina Wang1 · Chunhua Chen1 · Zhonghai Ren1 Received: 19 May 2020 / Accepted: 19 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message SlMYB75 increased the accumulation of JA and improved the scavenging of excess H2O2 to resist B. cinerea. Overexpression of SlMYB75 greatly prolongs tomato fruit storage life. Abstract Botrytis cinerea (B. cinerea) is a major threat to the production and storage life of tomato (Solanum lycopersicum) fruit around the world. SlMYB75 is an R2R3MYB transcription factor associated with the biosynthesis of anthocyanidin, but little is known about its function in the resistance of tomato to B. cinerea. In this study, we found that the overexpression of SlMYB75 regulated the accumulation of jasmonic acid (JA) and promoted the JA-mediated signaling pathway to resist B. cinerea infection. Moreover, the activities of peroxidase and superoxide dismutase, which were activated to scavenge hydrogen peroxide produced as a result of the B. cinerea infection, were enhanced in the transgenic tomato plants. Scanning electron microscopy images showed that the wax on the fruit skin surface was significantly decreased in the transgenic tomatoes compared with the wild type. However, SlMYB75 prolonged fruit storage life by both enhancing resistance to B. cinerea and directly downregulating the fruit shelf life-related gene SlFSR. Collectively, this study provides a good candidate gene for breeding high-quality tomatoes with a long storage life and high disease resistance. Keywords Tomato · SlMYB75 · Botrytis cinerea · Jasmonic acid · Fruit storage life
Introduction Tomato (Solanum lycopersicum) is an economically important vegetable crop worldwide and a key model plant for studying gene function and nutritional metabolism (Tieman et al. 2017; Zhang et al. 2019a, 2019b). However, tomato Communicated by Neal Stewart. Mengyu Liu and Zhen Zhang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02609-w) contains supplementary material, which is available to authorized users. * Zhonghai Ren [email protected] 1
State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang‑Huai Region, Ministry of Agriculture, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China
production faces a variety of problems, as it is seriously constrained by gray mold, which is one of the most serious tomato diseases and caused by B. cinerea (Botrytis cinerea) (Shah et al. 2012; Smith et al. 2014). Second, tomato is a soft fruit that loses its firm texture quickly during ripening (Zhang et al. 2018b). Therefore, toma
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