Oxytocin activates calcium signaling in rat sensory neurons through a protein kinase C-dependent mechanism
- PDF / 235,276 Bytes
- 6 Pages / 547.087 x 737.008 pts Page_size
- 0 Downloads / 182 Views
ORIGINAL PAPER
Oxytocin activates calcium signaling in rat sensory neurons through a protein kinase C-dependent mechanism Ahmet Ayar & Mete Ozcan & Ergul Alcin & Ihsan Serhatlioglu & Sibel Ozcan & Selim Kutlu & Haluk Kelestimur
Received: 30 October 2012 / Accepted: 18 July 2013 # University of Navarra 2013
Abstract In addition to its well-known effects on parturition and lactation, oxytocin (OT) plays an important role in modulation of pain and nociceptive transmission. But, the mechanism of this effect is unclear. To address the possible role of OT on pain modulation at the peripheral level, the effects of OT on intracellular calcium levels ([Ca2+]i) in rat dorsal root ganglion (DRG) neurons were investigated by using an in vitro calcium imaging system. DRG neurons were grown in primary culture following enzymatic and mechanical dissociation of ganglia from 1or 2-day-old neonatal Wistar rats. Using the fura-2-based calcium imaging technique, the effects of OT on [Ca2+]i and role of the protein kinase C (PKC)-mediated pathway in OT effect were assessed. OT caused a significant increase in basal levels of [Ca2+]i after application at the
doses of 30 nM (n=34, p
Data Loading...
