Ozonotherapy in an Induced Septic Shock. I. Effect of Ozonotherapy on Rat Organs in Evaluation of Free Radical Reactions
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Ozonotherapy in an Induced Septic Shock. I. Effect of Ozonotherapy on Rat Organs in Evaluation of Free Radical Reactions and Selected Enzymatic Systems Pawel Madej,1,6 Andrzej Plewka,2 Janusz A. Madej,3 Marcin Nowak,3 Danuta Plewka,4 Grzegorz Franik,1 and Darek Golka5
Abstract—The confirmed advantageous effects of oxygen/ozone therapy in several clinical conditions stimulated experimental studies on effects of the therapy in rats with an induced septic shock. The studies were conducted on adult male rats of Wistar strain. Four groups of the animals, each of 15 rats, included: IVcontrol group, (C); IIVanimals intraperitoneally administered with O2/O3 (CO), IIIVrats given of Escherichia coli endotoxin (lipopolysaccharideVLPS) (CL), IVVrats administered with the lipopolysaccharide plus administered with the oxygen/ozone mixture (OL). Activities of catalase and superoxide dismutase and of free radical reactions were estimated. The exposure to LPS augmented activities of SOD and of catalase in liver, lungs and heart. In all the examined organs LPS induced significant changes in levels of free radicals. Except of the lungs, parallel administration of the rats with LPS and ozone/oxygen revoked development of the alterations. The obtained results point to a strong, stabilizing and regenerative effect of ozonotherapy. KEY WORDS: septic shock; ozonotherapy; oxidative stress; free radicals; rats.
Septic shock is a sequele of infection with Gramnegative bacteria and frequently results in death of the host. Intravenous infusion of endotoxin provides the most popular experimental model of septic shock [2, 3]. Mediators of the shock development seem to include, i.a., reactive forms of oxygen. The oxygen/ozone therapy involves administration of O2/O3 gas mixture to body cavities, intraarterially, in the form of autohemotransfusion or under foil tents, etc. The intense supply of oxygen promotes supply of the gas to anoxic sites and, in parallel, inhibits development of bacteria, viruses and fungi, improving results of the principal therapy. Therapeutic doses of O2/O3 mixture activate enzymes responsible for body protection from processes linked to overproduction of superoxides [4, 5]. Ozone stimulates transmembraneous flow of oxygen, induces enzymes such as superoxide dismutase, catalase or peroxidases and makes oxygen utilization in the mitochondrial respiratory chain more effective.
INTRODUCTION Endotoxin represents the generally recognized agent which precipitates the chain of pathophysiological events leading to development of septic shock [1].
1
Department and Clinic of Gynaecological Endocrinology, Medical University of Silesia, ul. Medyko´w 14, 40-752, Katowice, Poland. 2 Department of Protein Chemistry and Enzymology, Medical University of Silesia, ul. Narcyzo´w 1, 41-200, Sosnowiec, Poland. 3 Department of Pathological Anatomy and Forensic Medicine, Veterinary Academy, ul. Norwida 31, 50-375, Wroclaw, Poland. 4 Department of Histology and Embryology, Medical University of Silesia, ul. Medyko´w 18, 40-752, Katowice, Po
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