Ozone Therapy in Induced Endotoxemic Shock. II. The Effect of Ozone Therapy Upon Selected Histochemical Reactions in Org
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Ozone Therapy in Induced Endotoxemic Shock. II. The Effect of Ozone Therapy Upon Selected Histochemical Reactions in Organs of Rats in Endotoxemic Shock Pawea Madej,1 Andrzej Plewka,2 Janusz A. Madej,3 Danuta Plewka,4 Wojciech Mroczka,1 Krzysztof Wilk,5 and Zuzanna Dobrosz6
Abstract—Mitochondria and lysosomes were evaluated by assessment of changes in activity of selected enzymes: lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), adenosinetriphosphatase (ATPase), acid phosphatase (AcPase) and b-glucuronidase (BG) in rats under profound hypoxia induced by endotoxemic shock. The study was conducted on adult male Wistar rats. The animals formed the following four groups of 15 rats each: control animals (C);Vrats receiving intraperitonally O2/O3 (CO), rats receiving of Escherichia coli toxin (LPS) (CL); rats receiving LPS plus oxygenYozone mixture (OL). Histoenzymatic examinations of liver, kidney, lungs, and heart muscle were performed. Lipopolysaccharide suppressed activities of all the enzymes except for LDH, the activity of which as high as a fourfold increase. The results demonstrated potent, stabilizing and regenerative effects of ozone therapy on body enzymatic processes in course of induced endotoxemic shock in rats, which might prove to be of clinical significance. KEY WORDS: endotoxemic shock; ozone therapy; oxidative stress; respiratory enzymes; rats.
in patients of high risk groups [1, 2]. Shock is a condition characterized by hypoxia, cellular malnutrition and a defective metabolic waste removal due to insufficient blood supply to the tissues. Aerobic metabolism turns into anaerobic one, which results in numerous changes including abnormalities in carbohydrate metabolism, generation of excessive amounts of lactate and the resultant metabolic acidosis. Pathological changes in various organs lead to imbalance between total oxygen supply and its utilization [3, 4]. The incidence of endotoxemic shock still remains high, which is liked to frequent use of immunosuppression, cytostatic drugs, wide spectrum antibiotics, and also increasing role of invasive diagnostic and therapeutic procedures [5, 6]. In gynecology and obstetric practice, septic shock develops usually in course of infections and less commonly as a postoperative complication. Inflammatory processes in internal organs frequently involve peritoneum. In addition to exogenous invasion and body immune responses, in the course of
INTRODUCTION Endotoxemic shock, due to a very high mortality reaching up to 60%, remains an important clinical issue
1
To whom correspondence should be addressed at Department and Clinic of Gynaecological Endocrynology, Medical University of Silesia, ul. Medyko´w 14, 40-752, Katowice, Poland. E-mail: pmadej@ slam.katowice.pl 2 Department of Protein Chemistry and Enzymology, Medical University of Silesia, ul. Narcyzo´w 1, 41-200, Sosnowiec, Poland. 3 Department of Pathological Anatomy and Forensic Medicine, Veterinary Academy, ul. Norwida 31, 50-375, Wrocaaw, Poland. 4 Department of Histology and Embryology, Medica
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