Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between
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Inflammation Research
ORIGINAL RESEARCH PAPER
Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines Chun Wang • Jun Yuan • Hua-xun Wu • Yan Chang • Qing-tong Wang • Yu-jing Wu • Li-hua Liu • Wei Wei
Received: 1 June 2013 / Accepted: 23 August 2013 / Published online: 5 October 2013 Ó Springer Basel 2013
Abstract Objective Paeoniflorin (Pae) was previously reported to inhibit inflammation in the skin of mice with allergic contact dermatitis (ACD); however, the mechanism remains unclear. The primary purpose of this study was to investigate the effect of Pae on the regulation of cytokine production in a murine model of ACD. Methods ACD was induced in the mice by repeated application of dinitrochlorobenzene (DNCB) to their skin. Cutaneous inflammation was evaluated by measuring ear swelling and by histological examination. The cytokine levels were measured by enzyme-linked immunosorbent assays. Results The results showed that topical application of DNCB caused obvious swelling and inflammatory cell infiltration. Treatment with Pae (70 or 140 mg/kg/d) significantly inhibited the cutaneous inflammation and decreased thymocyte proliferation in the mice with ACD. Additional data indicated that Pae increased interleukin-4 (IL-4) and IL-10 production but reduced IL-2 and IL-17 levels in the serum as well as in thymocyte and splenocyte culture supernatants. As expected, IL-2 and IL-17 levels in
Responsible Editor: Michael J. Parnham. C. Wang, J. Yuan, and H. Wu contributed equally to this work.
Electronic supplementary material The online version of this article (doi:10.1007/s00011-013-0662-8) contains supplementary material, which is available to authorized users. C. Wang J. Yuan H. Wu Y. Chang Q. Wang Y. Wu L. Liu W. Wei (&) Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China e-mail: [email protected]
the serum displayed a significant positive correlation with the severity of skin inflammation. In contrast, IL-4 and IL10 levels were negatively correlated with the inflammation. Conclusions The anti-inflammatory action of Pae in the murine model of ACD may be related to its regulation of an imbalanced cytokine production. Keywords Allergic contact dermatitis Paeoniflorin Interleukin-2 Interleukin-4 Interleukin-10 Interleukin -17
Introduction Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity reaction (DTH) mediated by hapten-specific T lymphocytes, which are primed in immune tissues during the sensitisation process (afferent phase). In this stage, numerous cytokines, such as interleukin (IL)-2, IL-4 and IL-17, are produced by T lymphocytes that have been activated by antigen-presenting cells (APC) [1]. A previous study concluded that contact hypersensitivity (CHS) in mice was promoted by an imbalance in secreted IL-2 and IL-4 [2]. Subsequently, excessive IL-17 an
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