Panax Notoginseng Saponins Inhibits Ventricular Remodeling after Myocardial Infarction in Rats Through Regulating ATF3/M
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C
hinese Journal of Integrative Medicine
• 897 •
Available online at link.springer.com/journal/11655 Journal homepage: www.cjim.cn/zxyjhen/zxyjhen/ch/index.aspx E-mail: [email protected]
Original Article
Panax Notoginseng Saponins Inhibits Ventricular Remodeling after Myocardial Infarction in Rats Through Regulating ATF3/MAP2K3/p38 MAPK and NFκB Pathway MA Ru-feng1,2, CHEN Guang2, LI Hong-zheng1,2, ZHANG Yun2, LIU Yong-mei2, HE Hao-qiang1,2, LIU Chen-yue1, XIE Zi-cong1,2, ZHANG Zhen-peng2, and WANG Jie2 Objective: To explore whether Panax notoginseng saponins (PNS) exhibits heart protective ABSTRACT Objective: Methods:: MI effect in myocardial infarction (MI) rats and to identify the potential signaling pathways involved. Methods rats induced by ligating the left anterior descending (LAD) coronary artery were assigned to sham coronary artery ligation or coronary artery ligation. Totally 36 Sprague-Dawley rats were randomly divided into sham group (distilled water, n =9), MI group (distilled water, n =9), PNS group (PNS, 40 mg/kg daily, n =9) and fosinopril group (FIP, 1.2 mg/kg daily, n =9) according to a random number table. The left ventricular morphology and function were conducted by echocardiography. Histological alterations were evaluated by the stainings of HE and Masson. The serum levels of C reactive protein (CRP), tumor necrosis factor α (TNF-α), growth differentiation factor-15 (GDF-15) and the ratio of metalloproteinase-9 (MMP-9) and tissue inhibitor of MMP-9 (TIMP-1) were determined by ELISA. The levels of activating transcription factor 3 (ATF3), mitogen-activated protein kinase kinase 3 (MAP2K3), p38 mitogen-activated protein kinase (p38 MAPK), phosphorylation of p38 MAPK (p-p38 MAPK), transforming growth factor-β (TGF-β1), collagen Ⅰ, nuclear factor kappa B p65 (NFκB p65), phosphorylation of NFκB p65 (p-NFκB p65), and phosphorylation of inhibitory kappa Bα (p-Iκ Bα) in Results:: PNS improved hearts were measured by Western blot and immunohistochemical staining, respectively. Results cardiac function and fibrosis in MI rats (P
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