Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons
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(2020) 21:831
RESEARCH ARTICLE
Open Access
Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons Ming Zhou, Ning Wang* , Gang Wang, Zishan Jia and Xiaolei Qi
Abstract Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs. Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LRPRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semiquantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis. Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14. Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs. Keywords: Platelet-rich plasma, Parecoxib, Nonsteroidal anti-inflammatory drugs, Tendinopathy
Background Platelet-rich plasma (PRP) is characterized by a high concentration of platelets, and is prepared by centrifuging autologous whole blood samples collected with anticoagulants. After activation, PRP is rich in autologous growth factors, including the transforming growth factor (TGF-β1), platelet-derived growth factor (PDGF), and vascular endothelial growth factor (VEGF). These growth factors have the potential to stimulate the * Correspondence: [email protected] Department of Rehabilitation Medicine, First Medical Center of Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, People’s Republic of China
proliferation of fibroblasts (PDGF) [1], collagen synthesis (TGF-β) [2], differentiation of fibroblasts and myoblasts (IGF) [3], while VEGF and hepatocyte growth factor (HGF) have a synergic effect on angiogenesis [4]. For these reasons, the use of PRP for sports injury treatment has gradually increased [5–7]. Previous studies on PRP use are conflicting; some showed favorable results [8, 9], while others failed to reproduce these outcomes [10, 11]. Variations of t
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