PCR for Clinical Microbiology An Australian and International Perspe
Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteri
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Margret Schuller · Theo P. Sloots · Gregory S. James · Catriona L. Halliday · Ian W.J. Carter Editors
PCR for Clinical Microbiology An Australian and International Perspective
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Editors Dr. Margret Schuller Profitable Business Development 1499 Sutherland NSW Australia [email protected] Gregory S. James Centre for Infectious Diseases and Microbiology ICPMR 2145 Westmead NSW Australia
Dr. Theo P. Sloots Sir Albert Sakzewski Virus Research Centre Queensland Children’s Medical Research Institute Herston Road 4029 Herston QLD Australia Catriona L. Halliday Centre for Infectious Diseases and Microbiology ICPMR 2145 Westmead NSW Australia
Ian W.J. Carter Prince of Wales Hospital SEALS Microbiology Department 2031 Randwick NSW Australia
ISBN 978-90-481-9038-6 e-ISBN 978-90-481-9039-3 DOI 10.1007/978-90-481-9039-3 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2010929696 © Springer Science+Business Media B.V. 2010 No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Foreword
The Polymerase Chain Reaction is a technique developed over 20 years ago. Like the preceding tools of nucleic acid hydridisation and DNA sequencing, it represented a breakthrough method that was almost instantly adopted as an indispensable tool in the biomolecular sciences. It is probably true to say that there is no organism that has been investigated by humans that has not had PCR applied to it to solve some basic aspect of its biology. The potential practical applications of the PCR were also recognized very early and its penetration into some industries is well known. Perhaps the most notable of these is in its application in forensic biology, a fact obvious in popular culture as can be seen from many prime time TV crime shows. The potential of PCR as a medical diagnostic tool was also recognized in the early days of PCR. Its uptake in this field has been relatively slow however and the early expectations are still to be realized. There are a number of reasons for this. Most of these however centre around the fact that its reliability and reproducibility to a standard required for clinical diagnosis is difficult to achieve. In a research laboratory, PCR is highly tailored to address specific research questions and time and resources are generally available to identify inconsistent data and trouble shoot where necessary. Clinical priorities however are driven by the need to have rigorously standardized protocols that can be consistently applied across diverse laboratories by personnel whose primary training is not necessarily in PCR. Apart from the tec
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