Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis
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ORIGINAL ARTICLE
Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis André Nobre Evangelista 1 & Flávia Franciele dos Santos 1 & Lorena Pacheco de Oliveira Martins 1 & Thais Peixoto Gaiad 1 & Alex Sander Dias Machado 2 & Etel Rocha-Vieira 2 & Karine Beatriz Costa 1 & Ana Paula Santos 1 & Murilo Xavier Oliveira 1 Received: 14 August 2020 / Accepted: 1 October 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract The aim of the present study was to investigate the effects of photobiomodulation (PBM) therapy on the expression of heat shock protein 70 (HSP70) and tissue repair in an experimental model of collagenase-induced Achilles tendinitis. Thirty Wistar rats (aged 12 weeks) were randomly distributed among control group (n = 8), tendinitis group (n = 11), and LED group (n = 11). Tendinitis was induced in the tendinitis and LED groups through a peritendinous injection of collagenase (100 μl). The LED group animals received the first irradiation 1 h after injury. A 630 ± 20 nm, 300-mW continuous wave light-emitting diode (LED), spot size 1 cm2, was placed in contact with the skin. One point over the tendon was irradiated for 30 s, delivering 9 J (9 J/ cm2). LED irradiation was performed once daily for 7 days, with the total energy delivered being 63 J. The tendons were surgically removed and expression of the HSP70 protein was calculated using semi-quantitative analyses of immunohistochemistry (HSCORE). Number of fibroblasts and amount of collagen were measured using histological and histochemical analyses. An increase in the mean HSCORE for HSP70, in the number of fibroblasts, and in the amount of collagen were found in the LED group compared with those in the tendinitis and control group (P ≤ 0.05). PBM therapy increased the expression of the HSP70, number of fibroblasts, and amount of collagen in the acute Achilles tendinitis in rats. Keywords Photobiomodulation therapy . Inflammation . HSP70 heat -shock proteins . Tendinopathy
Introduction Achilles tendinopathy is a clinical syndrome characterized by the combination of pain, swelling, and impaired performance. The multifactor etiology of tendinopathies is attributed to intrinsic factors, such as biomechanical changes, the advance in age and chronic health conditions, as well as extrinsic factors, such as excessive load on the Achilles tendon, direct mechanical injury, errors during training, and repetitive high loads [1, 2].
* Murilo Xavier Oliveira [email protected] 1
Department of Physical Therapy, Federal University of Jequitinhonha and Mucuri Valleys, UFVJM, Campus JK - MG Rodovia MGT 367 - Km 583, n° 5000 - Alto da Jacuba, Diamantina 39100-000, Brazil
2
School of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, UFVJM, Campus JK, Diamantina, MG, Brazil
Tendon healing occurs in three overlapping phases. First, there is an inflammatory stage, in which a clot forms, bringing platelets to the area, and an inflammatory response consisting of pred
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