Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study
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ORIGINAL ARTICLE
Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study Mariel Tavares Oliveira Prado Bergamo 1 & Luciana Lourenço Ribeiro Vitor 1 & Nathalia Martins Lopes 2 & Natalino Lourenço Neto 1 & Thiago José Dionísio 2 & Rodrigo Cardoso Oliveira 2 & Camila Oliveira Rodini 2 & Carlos Ferreira Santos 2 & Maria Aparecida Andrade Moreira Machado 1 & Thais Marchini Oliveira 1 Received: 29 July 2019 / Accepted: 31 January 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract This study evaluated the viability, proliferation, and protein expression after photobiomodulation (PBM) of stem cell from human exfoliated deciduous teeth (SHED). The groups were the following: G1 (2.5 J/cm2), G2 (3.7 J/cm2), and control (not irradiated). According to the groups, cells were irradiated with InGaAlP diode laser at 660 nm wavelength, continuous mode, and single time application. After 6 h, 12 h, and 24 h from irradiation, the cell viability and proliferation, and the protein expression were analyzed by MTT, crystal violet, and ELISA multiplex assay, respectively. Twenty-four hours after PBM, SHED showed better proliferation. Over time in the supernatant, all groups had an increase at the levels of VEGF-C, VEGF-A, and PLGF. In the lysate, the control and G2 exhibited a decrease of the VEGF-A, PECAM-1, and PLGF expression, while control and G3 decreased VEGF-C, VEGF-A, and PDGF expression. The dosimetries of 2.5 J/cm2 and 3.7 J/cm2 maintained viability, improved proliferation, and synthesis of the angiogenic proteins in the supernatant in the studied periods on SHED. Keywords Photobiomodulation therapy . Dental pulp . Angiogenic proteins . Stem cell
Introduction Tissue bioengineering involves the cellular and molecular study of different cell lineages, trigger signals of the cell differentiation, and scaffolds to lead the cells into the target tissue [1, 2]. For this purpose, stem cells have been used due to the clonogenic capacity, plasticity, and differentiation ability [3]. The differentiation into other cell types occurs according to the stimulus of the injured area, which is an important tool for tissue regeneration [ 4]. Mesenchymal stem cells from dental pulp have been studied with tissue engineering purposes [5–7].
* Thais Marchini Oliveira [email protected] 1
Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo 17012-901, Brazil
2
Department of Biology Science, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo 17012-901, Brazil
Stem cells are classified according to their origin into embryonary and adult mesenchymal stem cells. The dental pulp from both permanent and deciduous teeth has cells with phenotypic characteristic resembling adult mesenchymal stem cells, namely dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) [8–10]. Although DPSC and SHED are sources of undifferentia
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