Photobiomodulation with 630-nm LED radiation inhibits the proliferation of human synoviocyte MH7A cells possibly via TRP
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ORIGINAL ARTICLE
Photobiomodulation with 630-nm LED radiation inhibits the proliferation of human synoviocyte MH7A cells possibly via TRPV4/PI3K/AKT/mTOR signaling pathway Caiyun Meng 1 & Qing Xia 1 & Hao Wu 1 & He Huang 2 & Hailiang Liu 3 & Yujun Li 3 & Fengmin Zhang 3 & Wuqi Song 3 Received: 19 November 2019 / Accepted: 31 January 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract Phototherapy has been used to treat postoperative pain and inflammatory response in rheumatoid arthritis. Confidence in this approach, however, is impaired by lack of understanding of the light-triggered cellular and molecular mechanisms. The purpose of this study was to characterize the response of human synoviocyte MH7A cells to visible LED red light in an attempt to elucidate the associated action mechanism. Human synoviocyte MH7A cells were treated with 630-nm LED light after stimulation of tumor necrosis factor-α (TNF-α). The effects of light radiation on cell proliferation and migration were detected by MTT assay and scratch test. The expressions of inflammatory cytokines were measured using RT-qPCR. This was followed by detection of the levels of extracellular proteins IL-6 and IL-8 after differential radiation. Furthermore, the expression levels and activation of proteins on PI3K/AKT/mTOR signaling pathway were examined with Western blot. In terms of the proliferation and migration, repeated radiation with LED red light (630 nm, 26 and 39 J/cm2) exerted an inhibitory effect on synoviocyte MH7A cells. Expression of inflammatory factors (IL-6, IL-1β, IL-8, and MMP-3) was reduced; meanwhile, the expression of anti-inflammatory factor IL-10 was promoted. At the protein level, treatment with 39 J/cm2 of LED red light could decrease the level of extracellular protein (IL-6 and IL-8) and affect the expression and phosphorylation of proteins on TRPV4/PI3K/AKT/mTOR signaling pathway induced by TNF-α. These results demonstrated that LED red light (630 nm) inhibits proliferation and migration of MH7A cells. The growth-inhibiting effects of LED red light on human synoviocyte MH7A cells appear to be associated with regulation of the TRPV4/PI3K/AKT/mTOR signaling pathway. Keywords Proliferation . Synoviocyte . 630-nm LED . PI3K/AKT/mTOR pathway . TRPV4 channel
Introduction Rheumatoid arthritis (RA) is a systemic autoimmune disease that mainly affects joints, especially synovial joints. The major
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10103-020-02977-5) contains supplementary material, which is available to authorized users. * Wuqi Song [email protected] 1
Department of Microbiology, Wu Lien-Teh Institute, Harbin Medical University, Harbin, China
2
Department of Rheumatology and Immunology, the Second Affiliated Hospital of Harbin Medical University, Harbin, China
3
Department of Microbiology, the Heilongjiang Key Laboratory of Immunity and Infection, Harbin Medical University, Harbin, China
symptoms are chronic pain and swelling in multiple joints [1]
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