Physiological and Transcriptome Profiling Analyses Reveal Important Roles of Coronatine in Improving Drought Tolerance o
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Physiological and Transcriptome Profiling Analyses Reveal Important Roles of Coronatine in Improving Drought Tolerance of Tobacco Jiayang Xu1 · Yuyi Zhou1 · Zicheng Xu2 · Zheng Chen2 · Liusheng Duan1 Received: 5 April 2019 / Accepted: 14 January 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Coronatine (COR) is a phytotoxin produced by Pseudomonas syringae and is a functional analogue of the bioactive hormone JA-Ile, which is widely involved in plant defence responses. In this study, we explored the effects of exogenous applications of COR on tobacco plants under polyethylene glycol-induced drought stress. Compared with control (CK), COR-treated tobacco plants exhibited higher leaf relative water content and better photosynthetic performance under drought exposure. Ultrastructural examination revealed that drought led to stomatal closure and disorganization of granum stacking in the chloroplasts (with obvious accumulation of plastoglobuli), and mitochondria in the CK samples presented injured cristae. In the leaf tissue of the COR-treated plants, regularly stacked granum thylakoids, few plastoglobuli and intact mitochondrial membranes and cristae were observed. Totals of 1803 and 6207 differentially expressed genes (DEGs) were identified between the samples from the COR-treated and CK plants under well-watered and drought conditions. Functional annotation analysis revealed that these DEGs were involved mainly in plant hormone signal transduction, cellular carbohydrate metabolic processes and photosynthesis processes. Six hundred forty transcription factor genes were also identified among the DEGs. This study provides a global view of COR-induced drought stress tolerance in tobacco from both physiological and transcriptional aspects. Keywords Coronatine · Drought · Tobacco · Ultrastructural changes · Rna-seq
Introduction Drought stress and water scarcity are major constricting factors that severely limit crop growth and productivity (Mahajan and Tuteja 2005). In plants, exposure to drought stress may lead to a series of physiological and biochemical changes, including the direct suppression of alterations to the photosynthetic system, enhanced respiration, closure of leaf stomata and leaf senescence (Rivero et al. 2007; Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10074-8) contains supplementary material, which is available to authorized users. * Liusheng Duan [email protected] 1
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, People’s Republic of China
National Tobacco Cultivation and Physiology and Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, People’s Republic of China
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Chaves et al. 2009). In general, plant adaptations and resistance mechanisms to drought can be divided into escape, avoidance and tolerance strategies (Chaves et al. 2003). Phytohormones are internal molecules produced at low concentrat
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