A comprehensive integrated transcriptome and metabolome analyses to reveal key genes and essential metabolic pathways in
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ORIGINAL ARTICLE
A comprehensive integrated transcriptome and metabolome analyses to reveal key genes and essential metabolic pathways involved in CMS in kenaf Meiqiong Tang1,2 · Zengqiang Li1 · Dengjie Luo1 · Fan Wei1,2 · Muhammad Haneef Kashif1 · Hai Lu1 · Yali Hu1 · Jiao Yue1 · Zhen Huang1 · Wenye Tan1 · Ru Li3 · Peng Chen1 Received: 20 June 2020 / Accepted: 15 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Numbers of critical genes and pathways were found from the levels of transcriptome and metabolome, which were useful information for understanding of kenaf CMS mechanism. Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait in higher plants that leads to the inability to produce or release functional pollen. However, there is lack of comprehensive studies to reveal the molecular basis of CMS occurrence in kenaf. Herein, we performed transcriptome and UPLC-MS-based metabolome analyses in the anthers of a CMS (UG93A) and its maintainer (UG93B) to sort out essential genes and metabolites responding to CMS in kenaf. Transcriptome characterized 7769 differentially expressed genes (DEGs) between these two materials, and pathway enrichment analysis indicated that these DEGs were involved mainly in pentose and glucuronate interconversions, starch and sucrose metabolism, taurine and hypotaurine metabolism. In the metabolome assay, a total of 116 significantly different metabolites (SDMs) were identified between the CMS and its maintainer line, and these SDMs were involved in eight KEGG pathways, including flavone and flavonol biosynthesis, glycerophospholipid metabolism, flavonoid biosynthesis, glycosylphosphatidylinositolanchor biosynthesi. Integrated analyses of transcriptome and metabolome showed that 50 genes had strong correlation coefficient values (R2 > 0.9) with ten metabolites enriched in six pathways; notably, most genes and metabolites of flavonoid biosynthesis pathways and flavone and flavonol biosynthesis pathways involved in flavonoids biosynthetic pathways were downregulated in CMS compared to those in maintainer. Taken together, the decreased accumulation of flavonoids resulted from the compromised biosynthesis pathways coupled with energy deficiency in the anthers may contribute largely to CMS in UG93A of kenaf. Keywords Kenaf (Hibiscus cannabinus L.) · Cytoplasmic male sterility (CMS) · Transcriptome · Metabolome · Anther and pollen
Communicated by Neal Stewart. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02628-7) contains supplementary material, which is available to authorized users. * Peng Chen [email protected] 1
Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China
2
Guangxi Botanical Garden of Medicinal Plants, Guangxi Key Laboratory Resources Protection and Genetic Improvement, Nanning, China
3
College of Life Science and Technology, Guangxi University, Nanning, China
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