PIM1 inhibition attenuated endotoxin-induced acute lung injury through modulating ELK3/ICAM1 axis on pulmonary microvasc
- PDF / 1,366,306 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 117 Downloads / 177 Views
Inflammation Research
ORIGINAL RESEARCH PAPER
PIM1 inhibition attenuated endotoxin‑induced acute lung injury through modulating ELK3/ICAM1 axis on pulmonary microvascular endothelial cells Yumeng Cao1 · Xia Chen1 · Yuqi Liu1 · Xingyi Zhang1 · Yun Zou1 · Jinbao Li1 Received: 29 February 2020 / Revised: 26 September 2020 / Accepted: 1 November 2020 © Springer Nature Switzerland AG 2020
Abstract Objective The dysfunction of pulmonary microvascular endothelial cells (PMVECs) is one of the critical characteristics of acute lung injury/acute respiratory distress syndrome (ALI/ARDS) induced by severe infection. PIM1 is a constitutively active serine/threonine kinase that is involved in multiple biological processes. However, the underlying correlation between PIM1 and PMVECs injury remains unclear. The main purpose of this study was to reveal roles of PIM1 and explore the potential mechanisms during the development of endotoxin-induced ALI induced by intraperitoneal LPS administration. Materials and methods PIM1 level in the lung tissues of endotoxin-induced ALI mice or plasma derived from cardiopulmonary bypass (CPB)-induced ALI patients were measured. The protective roles of PIM1 specific inhibitor SMI-4a on endotoxin-induced lung injuries were evaluated through histological, permeability, neutrophil infiltration and survival assessment. The relationship between PIM1 and ELK3/ICAM-1 axis was validated in vivo and vitro. The correlation between plasma PIM1 and indicative vascular endothelium injury biomarkers (PaO2/FiO2 ratio, Ang-II, E-selectin and PAI-1) levels derived from CPB-induced ALI patient were analyzed. Results PIM1 expression in the lung tissues was increased in the mice of endotoxin-induced ALI. The PIM1 specific inhibitor SMI-4a administration relieved the severity of endotoxin-induced ALI. More importantly, PIM1 modulates ICAM1 expression through regulating transcription factor ELK3 expression in vitro. Eventually, plasma PIM1 level was positively correlated with Ang-II and PAI-1 levels but negatively correlated with SpO2/FiO2 ratio among CPB induced ALI patients. Conclusion Our results indicated that PIM1 inhibition carried a protective role against endotoxin-induced ALI by modulating the ELK3/ICAM1 axis on PMVECs. PIM1 may be a potential therapeutic target for endotoxin-induced ALI. Keywords PIM1 · Acute lung injury · Pulmonary microvascular endothelial cells · ELK3 · ICAM1
Introduction Responsible Editor: John Di Battista. Yumeng Cao and Xia Chen have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00011-020-01420-3) contains supplementary material, which is available to authorized users. * Yun Zou [email protected] * Jinbao Li [email protected] 1
Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20080, China
Sepsis is defined as life-threatening organ dysfunction caused by the host’s dysregulated inflammatory responses to se
Data Loading...
