Plant Cell and Tissue Culture - A Tool in Biotechnology Basics and A

This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading l

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Karl-Hermann Neumann • Ashwani Kumar Jafargholi Imani

Plant Cell and Tissue Culture - A Tool in Biotechnology Basics and Application

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Prof. Dr. Karl-Hermann Neumann Justus-Liebig-Universität Giessen Institut für Pflanzenernährung Heinrich-Buff-Ring 26-32 35392 Giessen, Germany Karl-Hermann.Neumann@ernaehrung. uni-giessen.de

Dr. Jafargholi Imani Justus-Liebig-Universität Giessen Institut für Phytopathologie und Angewandte Zoologie Heinrich-Buff-Ring 26-32 35392 Giessen, Germany [email protected]

Prof. Dr. Ashwani Kumar University of Rajasthan Department of Botany Jaipur 302004, India [email protected]

ISBN 978-3-540-93882-8

e-ISBN 978-3-540-93883-5

Principles and Practice ISSN 1866-914X Library of Congress Control Number: 2008943973 © 2009 Springer-Verlag Berlin Heidelberg Figures 3.2-3.5, 3.8, 3.10, 3.12, 3.13, 3.16, 4.1, 4.4, 5.2, 5.4, 5.5, 5.7, 6.3, 6.5, 6.6, 7.3, 7.5-7.9, 7.11, 7.15, 7.16, 7.33, 8.1, 8.3, 8.15, 9.2, 12.1, 13.3 and Tables 2.1, 3.3-3.8, 5.1, 6.1-6.3, 7.1, 7.3, 7.5, 7.8, 12.1 are published with the kind permission of Verlag Eugen Ulmer. This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permissions for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg, Germany. Cover illustration: Several stages of somatic embryos in carrot cell suspension. Printed on acid-free paper 9 8 7 6 5 4 3 2 1 springer.com

Preface

This book is intended to provide a general introduction to this exciting field of plant cell and tissue culture as tool in biotechnology, without overly dwelling on detailed descriptions of all aspects. It is aimed at the newcomer, but will hopefully also stimulate some new ideas for the “old hands” in tissue culture. Nowadays, with the vast amount of information readily available on the internet, our aim was rather to distill and highlight overall trends, deeming that a complete report of each and every tissue culture investigation and publication was neither possible, nor desirable. For some techniques, however, detailed protocols are given. We have tried to be as thorough as possible, and regret if we have inadvertently overlooked any pertinent literature or specific development that belong in this work. The three authors have been associated for many years, and have worked togethe