Plant Cell Culture Protocols
This fourth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniq
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Victor M. Loyola-Vargas Neftalí Ochoa-Alejo Editors
Plant Cell Culture Protocols Fourth Edition
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Cell Culture Protocols Fourth Edition
Edited by
Víctor M. Loyola-Vargas Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico
Neftalí Ochoa-Alejo Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico
Editors Víctor M. Loyola-Vargas Unidad de Bioquímica y Biología Molecular de Plantas Centro de Investigación Científica de Yucatán Mérida, Yucatán, Mexico
Neftalí Ochoa-Alejo Departamento de Ingeniería Genética Unidad Irapuato Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Irapuato, Guanajuato, Mexico
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8593-7 ISBN 978-1-4939-8594-4 (eBook) https://doi.org/10.1007/978-1-4939-8594-4 Library of Congress Control Number: 2018945533 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Plant cell, tissue, and organ culture techniques have been utilized for a long time and surely will continue to be important biological systems for a series of basic studies and also as biotechnological tools for clonal propagation of p
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