Plant Genome Editing with CRISPR Systems Methods and Protocols
This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiple
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Yiping Qi Editor
Plant Genome Editing with CRISPR Systems Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Genome Editing with CRISPR Systems Methods and Protocols
Edited by
Yiping Qi Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, MD, USA Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, USA
Editor Yiping Qi Department of Plant Science and Landscape Architecture University of Maryland College Park College Park, MD, USA Institute for Bioscience and Biotechnology Research University of Maryland Rockville, MD, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8990-4 ISBN 978-1-4939-8991-1 (eBook) https://doi.org/10.1007/978-1-4939-8991-1 Library of Congress Control Number: 2018965601 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The world has witnessed a great period of food crop productivity growth in the past 50 years. Notably, the introduction of crop genetic improvement technologies into the developing world has resulted in drastic yield increases for major staple crops such as wheat and rice. This achievement is remembered as the Green Revolution (1966–1985). Afterwards, recombinant DNA-based biotechnology contributed to the development of highly efficient genetically modified (GM) crops, thanks to pioneers like Mary-Dell Chi
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