Genome Editing in Animals Methods and Protocols
This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the hi
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Izuho Hatada Editor
Genome Editing in Animals Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Genome Editing in Animals Methods and Protocols
Edited by
Izuho Hatada Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan
Editor Izuho Hatada Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation Gunma University Maebashi, Gunma, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7127-5 ISBN 978-1-4939-7128-2 (eBook) DOI 10.1007/978-1-4939-7128-2 Library of Congress Control Number: 2017943506 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Conventional gene targeting is widely used to generate knockout mice. However, the conventional method depends on a rare event, spontaneous homologous recombination. This makes it impossible to produce gene-targeted mice directly from fertilized eggs, which in any case are limited in numbers for use in experiments. Consequently, for conventional gene targeting, embryonic stem cells (ESCs) are used instead of eggs because the former can be easily propagated. However, because generation of germline-competent ESCs is extremely difficult, conventional gene targeting is only possible for mice and rats, for which ESCs are available. This problem was solved
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