Plant-specific Dof transcription factors VASCULAR-RELATED DOF1 and VASCULAR-RELATED DOF2 regulate vascular cell differen
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Plant-specific Dof transcription factors VASCULAR-RELATED DOF1 and VASCULAR-RELATED DOF2 regulate vascular cell differentiation and lignin biosynthesis in Arabidopsis Vasagi Ramachandran1 · Yuki Tobimatsu2 · Yamamura Masaomi2 · Ryosuke Sano1 · Toshiaki Umezawa2,3 · Taku Demura1 · Misato Ohtani1,4 Received: 3 September 2019 / Accepted: 23 July 2020 © Springer Nature B.V. 2020
Abstract Key message Plant-specific Dof transcription factors VDOF1 and VDOF2 are novel regulators of vascular cell differentiation through the course of a lifetime in Arabidopsis, with shifting their transcriptional target genes. Abstract Vascular system is one of critical tissues for vascular plants to transport low-molecular compounds, such as water, minerals, and the photosynthetic product, sucrose. Here, we report the involvement of two Dof transcription factors, named VASCULAR-RELATED DOF1 (VDOF1)/VDOF4.6 and VDOF2/VDOF1.8, in vascular cell differentiation and lignin biosynthesis in Arabidopsis. VDOF genes were expressed in vascular tissues, but the detailed expression sites were partly different between VDOF1 and VDOF2. Vein patterning and lignin analysis of VDOF overexpressors and double mutant vdof1 vdof2 suggested that VDOF1 and VDOF2 would function as negative regulators of vein formation in seedlings, and lignin deposition in inflorescence stems. Interestingly, effects of VDOF overexpression in lignin deposition were different by developmental stages of inflorescence stems, and total lignin contents were increased and decreased in VDOF1 and VDOF2 overexpressors, respectively. RNA-seq analysis of inducible VDOF overexpressors demonstrated that the genes for cell wall biosynthesis, including lignin biosynthetic genes, and the transcription factor genes related to stress response and brassinosteroid signaling were commonly affected by VDOF1 and VDOF2 overexpression. Taken together, we concluded that VDOF1 and VDOF2 are novel regulators of vascular cell differentiation through the course of a lifetime, with shifting their transcriptional target genes: in seedlings, the VDOF genes negatively regulate vein formation, while at reproductive stages, the VDOF proteins target lignin biosynthesis. Keywords VASCULAR-RELATED DOF1 · VASCULAR-RELATED DOF2 · Vascular cell differentiation · Lignin · Vein patterning · Dof transcription factor · Arabidopsis thaliana Abbreviations 2D NMR Two-dimensional nuclear magnetic resonance BR Brassinosteroid Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01040-9) contains supplementary material, which is available to authorized users.
GUS β-Glucuronidase NAC NAC SECONDARY WALL THICKENING PROMOTING FACTOR PEAR PHLOEM EARLY DOF VDOF VASCULAR-RELATED DOF
* Taku Demura [email protected]
3
Research Unit for Development of Global Sustainability, Kyoto University, Uji, Gokasho, Kyoto 611‑0011, Japan
* Misato Ohtani [email protected]‑tokyo.ac.jp
4
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The Univ
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