Pocket Guide to Biomolecular NMR
Steering clear of quantum mechanics and product operators, "Pocket Guide to Biomolecular NMR" uses intuitive, concrete analogies to explain the theory required to understand NMR studies on the structure and dynamics of biological macromolecules. For examp
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Michaeleen Doucleff · Mary Hatcher-Skeers · Nicole J. Crane
Pocket Guide to Biomolecular NMR
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Dr. Michaeleen Doucleff National Institutes of Health (NIH) National Institute of Diabetes & Digestive & Kidney Deseases (NIDDK) Buildg.5 Center Drive 31 20892-0510 Bethesda Maryland USA [email protected]
Dr. Mary Hatcher-Skeers The Claremont Colleges WM Keck Science Center North Mills Avenue 925 91711 Claremont California USA [email protected]
Dr. Nicole J. Crane Henry M. Jackson Foundation for the Advancement of Military Medicine Naval Medical Research Center Dept. Regenerative Medicine Robert Grant Avenue 503 20910 Silver Spring Maryland USA [email protected]
ISBN 978-3-642-16250-3 e-ISBN 978-3-642-16251-0 DOI 10.1007/978-3-642-16251-0 Springer Heidelberg Dordrecht London New York © Springer-Verlag Berlin Heidelberg 2011 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
. . .energy is applied to the sample in the form of short, intense pulses, and nuclear signals are observed after the pulses are removed. The effects which result can be compared to the free vibration or “ringing” of a bell. . . Eric Hahn, 1953, Physics Today 6, 4–9
The essence of NMR is quite simple. You place a sample in a magnetic field, apply another field, and then collect the electromagnetic wave generated by the sample. No need to ionize, crystallize, burn, or even heat up the sample. A plain-vanilla solution of a compound in water is all you need to identify the substance and characterize many of its key biophysical properties. Unfortunately, the elegance and beauty of NMR is often lost when students open a textbook. Even the simplest introductions on NMR immediately immerse students in mathematics and theories laced with constants and concepts that lack tangible, macroscopic manifestations. For example, many textbooks begin by defining the energy of a nucleus in a magnetic field, E = −γmBo , a simple equation that is clear and understandable to all. However, take a closer look at this equality. Hidden inside the constants and variables are sophisticated concepts that are tough for
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