Polyimide Substrates Pre-Treated by Capacitively Coupled Plasma and Coated with Composites of Tetracycline-Imprinted Pol
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Polyimide Substrates Pre-Treated by Capacitively Coupled Plasma and Coated with Composites of Tetracycline-Imprinted Polymethacrylates and Quantum Dots as Fluorescent Sensors Ching-Bin Kea and Jian-Lian Chenb, * aDepartment
of Beauty and Health Care, Min-Hwei Junior College of Health Care Management, Tainan, 73658 Taiwan bSchool of Pharmacy, China Medical University, Taichung, 40402 Taiwan *e-mail: [email protected] Received January 17, 2018; revised April 3, 2018; accepted March 5, 2020
Abstract—After optimization of fabrication conditions, the capacitively coupled plasma-treated polyimide (PI) substrates were coated with composites of molecularly imprinted polymers and CdTe quantum dots and used to detect tetracycline (Tc) in the range of 5–3000 μM (R2 = 0.9995) with a LOD of 0.2 μM (S/N = 3, RSD = 2.2% at 5 μM Tc, n = 10) by using a fluorescent quenching effect. The selectivity of the stripped PIs was evaluated by the imprinting factors (IFs) for Tc (IF = 7.2), other Tc analogues (IF = 3.4–5.3), and steroids (IF ≈ 1) and by the recoveries of 5 μM Tc from bovine serum albumin at 300 μg/mL (98%, RSD = 3.2%, n = 5), fetal bovine serum at 1.5 ppt (98%, RSD = 2.8%, n = 5), and liquid milk (94.5%, RSD = 5.3%, n = 5). Keywords: imprinted polymer, plasma, polyimide, quantum dot, tetracycline DOI: 10.1134/S1061934820090063
Plastic substrates have been extensively developed for use in electrochemical sensors with advanced sensing materials and fabrication techniques, such as substrate pretreatment, film deposition, line patterning, and module assembly at low temperatures that a plastic substrate can sustain [1–3]. Many of the electronic noses based on plastic plates aim at gas detection to avert false connections between the conductive elements in solution. In contrast, electrodeless optical sensors are rarely based on plastic substrates but are instead mostly applied to aqueous bioassays. Without the restriction on the transparency of substrates required for colorimetric assays [4], fluorometric assays have allowed the immobilization of antibodies [5, 6], proteins [7], and DNA [8] via passive adsorption [5], photocrosslinkable hydrogels [7], and covalent bonding [6, 8], respectively, on the plastic surfaces to detect fluorophore-labeled targets. Polyimide (PI) is one of the most used materials as a plastic substrate. Due to its inertness, certain treatments, such as electron beam [9], laser ablation [10], ion beam [11], UV/O3 radiation [12], gamma irradiation [13], plasma [14–18], and chemicals [19–21], have been applied to PI substrates to improve the adhesion of coating materials. In contrast to the dry treatments, wet chemical modification possesses lower instrument expense but is a more tedious procedure. Plasma drying is the most prevalent process among the dry-type options. For example, air [14], He
[15], N2 [16], O2 [17], H2 [18], and Ar [17, 18] plasmas are favorable for Ti [14], Ag [15], Cu [16, 18], and Pt [17] coatings. In addition to a few studies on the precursor film deposition [22, 23], physic
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