Polymer Films of Poly-3-hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources
- PDF / 2,588,642 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 50 Downloads / 161 Views
ORIGINAL PAPER
Polymer Films of Poly‑3‑hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources Ekaterina Shishatskaya1,2 · Ivan Nemtsev3 · Anna Lukyanenko3 · Alexander Vasiliev1,3 · Evgeniy Kiselev1,2 · Aleksey Sukovatyi1,2 · Tatiana Volova1,2 Accepted: 7 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by the Cupriavidus necator B-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrate influenced molecularweight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced Mw (300–400 kDa) and degree of crystallinity (50–55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness ( Ra) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4–1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4°, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7–1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose. Keywords Degradable P(3HB) · Various carbon substrates · Films · Structure · Properties · NIH 3T3 fibroblasts
Introduction Development of new environmentally friendly materials, which will completely degrade without releasing toxic products, joining the global cycles, should be the priority for critical technologists of the twenty-first century. Annual production of synthetic plastics has exceeded 360 million tons, and they largely accumulate in the biosphere, posing a global environmental threat [1, 2]. Therefore, considerable research effort has been devoted recently to the production of newgeneration biodegradable materials for various applications,
* Tatiana Volova [email protected] 1
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, Russia 660041
2
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia
3
L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russia
which is in line with the concept of safe sustainable industrial development. There is a specific demand for biocompatible materials for biomedical applications, to be used in contact with tissues of human body. Improvement of the methods of regenerative medicine based on tissue engineering is impossible without creating new, highly functional and specific,
Data Loading...
