Polymorphic microsatellite markers developed from 454 pyro-sequencing in the cold water coral-associated squat lobster s
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TECHNICAL NOTE
Polymorphic microsatellite markers developed from 454 pyro-sequencing in the cold water coral-associated squat lobster species, Eumunida picta (Chirostylidae: Eumunididae) D. K. Coykendall • C. L. Morrison
Received: 26 November 2012 / Accepted: 10 December 2012 / Published online: 11 January 2013 Ó Springer Science+Business Media Dordrecht (outside the USA) 2013
Abstract Eighty-three putative microsatellite loci were screened from two 454 shotgun genomic libraries from Eumunida picta (Chirostylidae:Eumunididae), a species of squat lobster closely associated with cold-water coral reefs. Sixteen loci amplified consistently and nine proved sufficiently polymorphic and in Hardy Weinberg equilibrium in one or both sampling sites from Vioska Knoll 826 in the Gulf of Mexico and off of Cape Lookout, North Carolina. The average number of alleles per locus was 17.3 and observed heterozygosity ranged from 18.8–93.3 % with an average of 64.4 %. These genetic markers will be utilized to determine genetic connectivity among cold water coral reef communities of E. picta off the coast of the southern continental US and in the Gulf of Mexico. This will be the first attempt to examine E. picta population differentiation within and between fragmented reef habitats. Keywords Microsatellite Eumunida picta Squat lobster
Cold water coral (CWC) ecosystems within the deep sea are considered biodiversity hotspots (Roberts et al. 2006). CWC reefs occur throughout the Atlantic and Pacific Oceans along continental margins and seamounts in regions increasingly exploited as a source of minerals, petroleum, and commercially valuable groundfish. Using genetic markers as a tool to characterize the genetic connectivity between discrete habitats helps to elucidate how immigration, larval recruitment and settlement, and long D. K. Coykendall (&) C. L. Morrison United States Geological Survey Leetown Science Center, 11649 Leetown Rd, Kearneysville, WV 25430, USA e-mail: [email protected]; [email protected]
term persistence of populations and communities survive. In the western North Atlantic Ocean, the squat lobster, Eumunida picta (Eumunididae), is an abundant invertebrate, a dominant scavenger and predator, and possible source of food from these habitats to other deep sea communities, associated with Lophelia reefs (Baba et al. 2008; Kilgour and Shirley 2008; Poore et al. 2011). These genetic markers will be used to examine E. picta population connectivity and compare genetic differentiation patterns with its coral host. Eumunida picta individuals were collected using the Johnson-Sea-Link submersible (Harbor Branch Oceanographic Institute), Jason2 remotely operated vehicle (ROV, Woods Hole Oceanographic Institute), the Kraken ROV (University of Connecticut), and an otter trawl deployed from the R/V Cape Hatteras from Cape Canaveral (CCN), Florida and Cape Lookout (CLO), North Carolina in the Atlantic (ATL) and Mississippi Canyon (MC751), Garden Banks (GB535), West Florida Slope (WFL), and Vioska Knoll (VK826) in the Gul
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