Preparation and Characterization of Glued Corn Flakes-Like Protein-Based Magnetic Particles
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ORIGINAL ARTICLE
Preparation and Characterization of Glued Corn Flakes‑Like Protein‑Based Magnetic Particles Waisudin Badri1 · Mohamad Tarhini1 · Zineb Lgourna2 · Noureddine Lebaz1 · Hassan Saadaoui3 · Nadia Zine4 · Abdelhamid Errachid4 · Abdelhamid Elaissari1 Received: 27 January 2020 / Accepted: 14 May 2020 © The Tunisian Chemical Society and Springer Nature Switzerland AG 2020
Abstract Purpose The aim of this study was to prepare and to characterize bovine serum albumin protein-based magnetic nanoparticles in terms of chemical composition (weight loss of organic material), physicochemical and colloidal properties. Methods Bovine serum albumin-based iron oxide colloidal particles were prepared by coprecipitation of ferrous chloride (FeCl2), ferric chloride ( FeCl3) in highly basic medium and in the presence of protein at high temperature. The particle morphology was examined using both Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM) whereas particle size, size distribution and zeta potential were obtained by Dynamic Light Scattering (DLS). The organic matter mass loading was revealed by Thermogravimetric Analysis (TGA). Results The particle size was found to be submicronic, which confers to such magnetic colloids a low sedimentation rate. Their morphology was non-spherical and non-smooth in nature but glued corn flakes-like, which reflects the heterocoagulation mechanism process involved in the formation of the particles. The designed particles contain about 73% w/w iron oxide, whereas only 50% w/w was determined by magnetization. Conclusion Proteins as promising crosslinking agents to prepare hybrid particles. Keywords Hybrid particles · Protein · Crosslinking · Magnetic nanoparticles · Particle size · Size distribution
1 Introduction Based on new estimations, 10% (more than US$40 billion/ year) or over 100 approved peptide-based therapeutics of the pharmaceutical market are peptide and protein drugs. Peptides and proteins (i.e., enzymes and antibodies) are highly selective thanks to their multiple target contacts in compare to the typical small drug molecules, which form * Waisudin Badri waisudin.badri@univ‑lyon1.fr 1
University of Lyon, University Claude Bernard Lyon-1, CNRSLAGEPP-UMR 5007, 43 Boulevard du 11 Novembre 1918, 69100 Villeurbanne, France
2
Labomine Laboratory, Lot No. 35, Ibnou Rochd, Z.I Tassila, 80000 Agadir, Morocco
3
Centre de Recherche Paul Pascal, Université de Bordeaux, 33600 Pessac, France
4
UMR 5280, Institut Des Sciences Analytiques, University of Lyon, CNRS, University Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France
the large part of the pharmaceutical market [1]. Different types of proteins were employed like building blocks in the delivery of small molecules that attract much attention thanks to their unique properties including biocompatibility, biofunctionality, and biodegradability. Indeed, proteins such as gelatin, albumin, gliadin, zein could transport drugs over the blood brain barrier [2]. Nanoparticles a
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