Preparation of amino-functionalized silica supports for immobilization of epoxide hydrolase and cutinase: characterizati
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Preparation of amino‑functionalized silica supports for immobilization of epoxide hydrolase and cutinase: characterization and applications Manoj Kamble1 · Harshada Salvi1 · Ganapati D. Yadav1
© Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Mesocellular foam silica (MCF) and Santa Barbara Amorphous (SBA-15) were synthesized using the hydrothermal method and then amino-functionalized to immobilize two enzymes: epoxide hydrolase and cutinase. In order to achieve high stability and to maintain the activity of enzymes, the supports were surface-functionalized, and the glutaraldehyde cross-linking method was employed for further entrapment of the enzyme. Epoxide hydrolase was partially purified from red mung beans, while cutinase was produced by Fusarium sp. ICT SAC1 and used for immobilization. The developed biocatalysts were well characterized using XRD, SEM, TEM, FTIR, BET, TGA techniques and successfully employed for some reactions such as the chiral resolution of (R,S)-phenyl glycidyl ether, (R,S)-styrene oxide, (R,S)-phenyl ethanol and acylation of geraniol. Keywords Mesoporous silica · Amino functionalization · Cutinase · Epoxide hydrolase · MCF · SBA-15
1 Introduction Biotransformation is nothing but the use of suitable microbes, cells, or enzymes to catalyze a chemical reaction in a selective manner, which yields commercially valuable products and intermediates in the pharmaceutical, chemical, and agrochemical industries. Presently, biocatalysis is one of the suitable alternatives to traditional chemical processes concerning green and sustainable chemistry [1]. Enzymology deals with the development of the “ideal” biocatalyst for industrial use at large scale. However, the use of enzymes is restricted by their own properties, such as their low operational stability and reusability. To overcome these problems, immobilization of enzyme on a suitable support paves the Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10934-020-00931-y) contains supplementary material, which is available to authorized users. * Ganapati D. Yadav gd.yadav@ictmumbai.edu.in; gdyadav@yahoo.com Manoj Kamble mpkkamble@gmail.com Harshada Salvi ce14hm.salvi@pg.ictmumbai.edu.in 1
Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, India
way to enhance operational stability (thermal, chemical, mechanical, and pH stability) as well as reusability [1–3]. In this context, nanotechnology plays a critical role in the development of clean and green technologies [4–6]. Recently, ordered mesoporous materials have been intensively used. The mesoporous class of materials constitutes of many materials, mainly silica, carbon structures, various oxides and hydroxide of metal, metal salts, etc. [7]. The synthesis of ordered siliceous materials such as mesocellular silica foam (MCF) and Santa Barbara Amorphous (SBA-15) has started a new era in material science and engineering. These mesoporous materials
