Functionalized hierarchical wrinkled-silica spheres for laccases immobilization
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Functionalized hierarchical wrinkled‑silica spheres for laccases immobilization Keyla M. Fuentes1 · Lucy L. Coria‑Oriundo2,3 · Sonia Wirth4 · Sara A. Bilmes2,3 Accepted: 18 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Functionalized mesoporous S iO2 are common supports for some enzymes of industrial interest, such as laccases. However, the incorporation of specific functionalities and the loading of enzymes with dimensions close to the diameters of the pores obstructs the porous system. For biotechnological applications, tailored porous supports are still needed to enhance the laccase loading. The hierarchical meso/macroporous system in wrinkled-SiO2 spheres (w-SiO2) is a suitable option to overcome this issue. Herein, (3-aminopropyl) triethoxysilane and glutaraldehyde were use as functionalizing agents for the immobilization of laccase on w-SiO2. The functionalization occurs in the mesopores of the wrinkled walls and the preservation of the macroporous entries facilitates the diffusion of the laccase inside the particle. The enzyme performance was evaluated by means of the crystal violet bleaching. The enzyme is stabilized through the imine groups provided by glutaraldehyde, allowing the retention of the activity after several reaction cycles. The bleaching can be boosted by acetosyringone, highlighting the possibility of using redox mediators to expand the range of oxidizable substrates. Understanding the effect of w-SiO2 functionalization on laccases loading and performance could be extrapolated to other enzymes with biotechnological interest that requires this type of hierarchical porous silica. Keywords Hierarchical porous silica · Silica functionalization · Enzyme immobilization
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10934-020-00988-9) contains supplementary material, which is available to authorized users. * Keyla M. Fuentes [email protected] * Sara A. Bilmes [email protected] 1
Centro de Investigación en Materiales Avanzados, S. C. (CIMAV), Unidad Monterrey, Parque de Investigación e Innovación Tecnológica, Alianza Norte 202, 66628 Apodaca, Nuevo León, México
2
CONICET – Universidad de Buenos Aires, Instituto de Química Física de los Materiales, Medio Ambiente y Energía‑ INQUIMAE, Pza. Gregorio Klimovsky, Ciudad Universitaria, Universidad de Buenos Aires, Pabellón 2, C1428EHA Ciudad de Buenos Aires, Argentina
3
Departamento de Química Inorgánica, Analítica y Química Física, DQIAQF, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, FCEyN, Ciudad de Buenos Aires, Argentina
4
Laboratorio de Agrobiotecnología, DFBMC-FCEN-UBA and IBBEA, CONICET-UBA, C1428EGA, Ciudad de Buenos Aires, Argentina
1 Introduction Enzymes immobilization onto mesoporous SiO2 is a common strategy for the implementation of biotechnological processes at industrial level mainly due to the thermal and mechanical stability and large surface areas of these materials [1–4
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