Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats
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Journal of Nanobiotechnology Open Access
RESEARCH
Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study Payam Khazaeli1,2, Maryam Alaei3, Mohammad Khaksarihadad4 and Mehdi Ranjbar1*
Abstract Diabetes mellitus is one of the most common metabolic disorders. One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound. The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing. In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute. After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV−Vis spectra. The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis. The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects. According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome. Keywords: Poly lactic acid/chitosan nanoscaffolds, Cod liver oil, Electrospinning, Diabetic foot ulcer Research highlights a. Poly lactic acid/chitosan nanoscaffolds were synthesized using microwave-assisted electrospinning process. b. Nanoscaffolds showed high potential in wound healing recovery after 14 days. c. PLA/chitosan nanoscaffolds containing 30% cod liver oil were synthesized with the size of about 50–150 nm. *Correspondence: [email protected] 1 Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, P.O. Box: 76175‑493, Kerman 76169‑11319, Iran Full list of author information is available at the end of the article
d. Wound area indicated that there was significant improvement in wound surface on the 14th day.
Introduction The global prevalence of diabetes has increased dramatically over the past 2 decades [1]. Diabetes mellitus is the most common heterogeneous metabolic disorder [2], which is associated with a disorder in the metabolism of sugars, lipids, and proteins and is characterized by elevated blood glucose or insulin response to tissues [3, 4]. Patients suffering from diabetes mellitus have limited ability to stimulate the immune response and are very susceptible to infection and at the risk of terminal li
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