Effects of resveratrol on incisional wound healing in rats
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ORIGINAL ARTICLE
Effects of resveratrol on incisional wound healing in rats Ismail Yaman • Hayrullah Derici • Cemal Kara Erdinc Kamer • Gulden Diniz • Ragip Ortac • Oya Sayin
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Received: 27 July 2011 / Accepted: 26 August 2012 Ó Springer Japan 2012
Abstract Purpose The objective of this study was to investigate the effect of resveratrol on the healing process after midline laparotomy in rats. Methods The study was performed on adult female Wistar-Albino rats. The study group was orally administered 0.5 mg/kg resveratrol once a day for 7 days before the operation until 12 h before surgery and then the treatment was maintained throughout the study. Each rat was anesthetized, and a 4-cm midline laparotomy was performed. Ten animals in each group were sacrificed on postoperative days 7, and 14. A tensile strength analysis was performed, hydroxyproline levels were measured, and the abdominal incision wounds were examined histologically.
Results Resveratrol administration significantly increased the tensile strength of the abdominal fascia, and increased the hydroxyproline levels on postoperative day 14. The acute inflammation scores, collagen deposition scores and the neovascularization scores on postoperative days 7 and 14 were found to be significantly higher in the resveratrol treatment group compared to the control group. The amount of granulation tissue and the fibroblast maturation scores were found to be significantly higher only on postoperative day 14 in the treatment group compared to the control group. Conclusion Our findings show that resveratrol may have a beneficial effect on incisional wound healing. Keywords Hydroxyproline Incisional wound healing Nitric oxide Resveratrol Tensile strength
I. Yaman (&) H. Derici Department of General Surgery, The School of Medicine, Balikesir University, Ataturk mah Bandirma Cad 47/6, Balikesir, Turkey e-mail: [email protected] C. Kara Department of General Surgery, Karsiyaka State Hospital, Izmir, Turkey E. Kamer Department of General Surgery, Ataturk Training and Research Hospital, Izmir, Turkey G. Diniz R. Ortac Department of Pathology, Dr. Behcet Uz Children’s Hospital, Izmir, Turkey O. Sayin Research Laboratory of Biochemistry, Department of Biochemistry, The School of Medicine, Dokuz Eylul University, Izmir, Turkey
Introduction Wound healing is a natural restorative response to tissue injury. It is a complex and dynamic process consisting of three phases: inflammation, proliferation, and maturation and remodeling [1, 2]. The proliferative process, which begins within days of injury includes the major processes of healing. It is characterized by angiogenesis, collagen deposition, granulation tissue formation, epithelialization and wound contraction [1–3]. Angiogenesis promotes the tensile strength of the wound, and plays a major role in tissue repair [3, 4]. Vascular endothelial growth factor (VEGF) is a potent and specific mediator for vascular endothelial cells and, this molecule plays a unique role in the regulation of physiological angiog
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