Pretreatment of Mango ( Mangifera indica L. Anacardiaceae) Seed Husk for Bioethanol Production by Dilute Acid Treatment
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Pretreatment of Mango (Mangifera indica L. Anacardiaceae) Seed Husk for Bioethanol Production by Dilute Acid Treatment and Enzymatic Hydrolysis Francis Dave C. Siacor, et al. [full author details at the end of the article] Received: 5 December 2019 / Accepted: 16 July 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
One of the targets of the Sustainable Development Goals is clean and affordable energy. This is also the aim of the Biofuels Act of 2007 in the Philippines. However, this law is confronted with challenges such as the limitation of lignocellulosic feedstock, specifically available for bioethanol production. The present study sought to address the issue by exploring the potential of mango seed husk (MSH), a by-product of the mango industry, in bioethanol production. MSH is considered a waste material and its utilization also permit value-addition as this can serve as an alternative and affordable source of feedstock in energy production. Two pretreatment strategies are employed to exploit the cellulose and hemicellulose content of MSH, namely, dilute acid treatment and enzymatic hydrolysis. Results show that the %H2SO4 resulting in the highest glucose concentration and yield is 4% v/v at 95 °C hydrolysis temperature, 1:10 (w/v) solid-to-solvent ratio, and 60-min hydrolysis time. For enzymatic hydrolysis using a commercial enzyme preparation, the reaction time up to 72 h did not affect glucose concentration and yield at the following conditions: 50 °C hydrolysis temperature, 150 rpm, pH 5.0, 10% solids loading, and 4% enzyme loading. This could be attributed to the lignin and non-structural compounds present in MSHs. However, a combined process strategy of dilute acid pretreatment followed by enzymatic hydrolysis in the pretreatment of MSH contributes to an increased concentration and yield of sugars in the hydrolysates, which is advantageous for bioethanol production.
Keywords Bioethanol . Dilute acid treatment . Enzymatic hydrolysis . Lignocellulosic biomass . Mango seed husk . Pretreatment strategies Highlights • Mango seed husk as a lignocellulosic material is a potential feedstock for bioethanol production. • Chemical and enzyme-assisted pretreatment of mango seed husk generated sugar-rich hydrolysates. • Dilute acid pretreatment using 4% H2SO4 at 95 °C for 1 h produces sugar-rich hydrolysates with minimum inhibitor content. • Enzyme hydrolysis using a cellulase mixture at 50 °C for up to 72 h resulted in a hydrolysate having optimal sugar concentration. • The combination of both dilute acid and enzymatic hydrolysis in the pretreatment process of mango seed husks significantly increased the sugar concentration of the resulting hydrolysates. The presenting author in ACB2019 is F.D.C.S.
Applied Biochemistry and Biotechnology
Introduction The Sustainable Development Goal (SDG) No. 7 specifies to target “Clean and Affordable Energy.” In the Philippines, the Biofuels Act (Republic Act 9367) in 2007 mandated 10% and 5% ethanol and biodiesel blends in transport fuel,
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