Assessment of multiple pretreatment strategies for 2G L-lactic acid production from sugarcane bagasse
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ORIGINAL ARTICLE
Assessment of multiple pretreatment strategies for 2G L-lactic acid production from sugarcane bagasse Ketaki Nalawade 1 & Paharika Saikia 1 & Shuvashish Behera 1 & Kakasaheb Konde 1 & Sanjay Patil 1 Received: 13 August 2020 / Revised: 12 November 2020 / Accepted: 18 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The bioprospecting of sugarcane bagasse (SCB) through alkali, acid, and hydrodynamic cavitation pretreatment methods and their combinations were evaluated based on bagasse composition, enzymatic hydrolysis, and lactic acid productivity using Bacillus coagulans NCIM 5648. From 100.0 g of SCB, L-lactic acid production of 26.16 g, 8.78 g, 14.15 g, 14.33 g, and 24.61 g in alkali, acid, sequential acid-alkali, sequential alkali-acid, and cavitation with alkali pretreatment was obtained, respectively. Considering the holistic approach from SCB to L-lactic acid, alkali pretreatment is found to be the best method with L-lactic acid titer of 68.7 g/L, the productivity of 2.86 g/L/h, and yield of 0.92 g/g which has resulted in 82.5% higher product yield from SCB as compared to alkali-acid pretreatment. Cavitation in presence of alkali evolved as the next better route with L-lactic acid titer of 62.5 g/L, the productivity of 2.60 g/L/h, and yield of 0.92 g/g. Though the highest glucose release of 89.3 g/L was achieved during enzymatic hydrolysis with sequential alkali-acid-pretreated SCB that resulted in the highest Llactic acid titer of 71.8 g/L, the productivity of 2.99 g/L/h and fermentation yield of 0.90 g/g. Keywords Sugarcane bagasse . Pretreatment . Enzymatic hydrolysis . L-lactic acid . Bacillus coagulans
1 Introduction Lactic acid has numerous applications in food, chemical, textile, pharmaceutical, and other industries [1, 2]. It is estimated that the global demand for lactic acid will increase from 1220 kt in 2016 to 1960 kt by 2025 [3]. It can be produced commercially either chemically or by fermentation. Chemical synthesis results in a racemic mixture of two isomers. However, microbial fermentation can lead to an optically pure isomer depending on the strain, raw materials, and conditions used during fermentation [3, 4]. Besides, fermentative lactic acid production is a greener technique as compared to chemical synthesis due to limited use of harsh chemicals, mild operating conditions (lower reaction temperature and pressure), lesser byproducts, etc. [5]. Lignocellulosic biomass (LCB) is one of the most readily available renewable sources of energy. It can be utilized to generate various products ranging from biopower to biofuels, * Sanjay Patil [email protected] 1
Department of Alcohol Technology and Biofuels, Vasantdada Sugar Institute, Manjari (Bk.), Pune 412307, India
biobased bulk chemicals, and specialty products [6]. Sugarcane bagasse (SCB) is the by-product of the sugar industry with a well-established supply chain. It is the choice of the substrate because it was one of the surplus agro-industrial wastes in the year 2018–2019 in In
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