Probiotic Lactobacillus johnsonii BS15 Prevents Memory Dysfunction Induced by Chronic High-Fluorine Intake through Modul
- PDF / 3,033,688 Bytes
- 19 Pages / 595.276 x 790.866 pts Page_size
- 28 Downloads / 173 Views
Probiotic Lactobacillus johnsonii BS15 Prevents Memory Dysfunction Induced by Chronic High-Fluorine Intake through Modulating Intestinal Environment and Improving Gut Development Ning Sun 1 & Xueqin Ni 1 & Hesong Wang 2 & Jinge Xin 1 & Ying Zhao 1 & Kangcheng Pan 1 & Bo Jing 1 & Dong Zeng 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In recent years, the influence of chronic fluorosis on the brain has been widely reported. Our study aimed to demonstrate the potential mechanism underlying the impairment of memory function by excessive fluorine intake. We also evaluated whether improvement of intestinal microflora could be a potential therapy to prevent the negative influences from the perspective of gut-brain axis. Male ICR mice were randomly divided into three groups and administered with either phosphate buffered saline (PBS) (Control and F groups) or Lactobacillus johnsonii BS15 (FP group; daily amounts of 1 × 109 CFU/mL), a probiotic strain, by oral gavage throughout a 98day experimental period. Sodium fluoride (100 mg/L) was added to the drinking water of the F and FP groups. Animals were sacrificed for sampling with or without water avoidance stress (WAS) at two phases of the experiment and behavioral tests including T-maze test and passive avoidance test were also performed. Based on the results of behavioral tests, probiotic reversed the fluorineinduced memory dysfunction. In addition, L. johnsonii BS15 also increased the antioxidant capacities (serum and hippocampal tissue) and hippocampal synaptic plasticity-related mRNA expression after excessive fluoride ingestion. Moreover, the increased colonization of L. johnsonii BS15 also protected the small intestines from the damages of growth performance, visceral indexes, intestinal development, digestive, and secretory functions by changing the structure of the microflora and then improving intestinal permeability and integrity. L. johnsonii BS15 also improved the ability of flourosis mice against psychological stress indicated by the changes in behavioral tasks, hippocampal antioxidant levels, and synaptic plasticity-related mRNA expressions. Lactobacillus johnsonii BS15 intake appears as a promising way to ameliorate fluorine-induced memory dysfunction, especially under psychological stress. Keywords Fluorosis . Gut-brain axis . Memory impairment . Probiotic . Psychological stress
Introduction Fluorine is one of the trace elements to maintain the organisms’ health. It is also an essential component for normal Ning Sun and Xueqin Ni are equal contributors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12602-020-09644-9) contains supplementary material, which is available to authorized users. * Hesong Wang [email protected] * Dong Zeng [email protected] 1
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China
2
Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guang
Data Loading...
