Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel res
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ORIGINAL ARTICLE
Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues A. Valdez-Calderón 1 & M. Barraza-Salas 2 & M. Quezada-Cruz 3 S. Carrillo-Ibarra 4 & M. Rodríguez 5 & N. G. Rojas-Avelizapa 6
M. A. Islas-Ponce 1,2 & A. F. Angeles-Padilla 1 & 3 1 & A. Garrido-Hernández & A. M. Rivas-Castillo
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Received: 26 May 2020 / Revised: 5 November 2020 / Accepted: 13 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Plastics are widely used for various applications. Once discarded, it is commonly known that they represent a high environmental threat due to their slow degradation; for this reason, there is an imminent need to replace these products with eco-friendlier ones. In the present work, four bacterial polyhydroxybutyrate (PHB) producers, two consortia, and two isolated strains were successfully recovered from the facilities of a paper-manufacturing industry. Spectroscopic studies of the biopolymers obtained from these bacteria corroborated their PHB production capabilities, ranging from 4.04 ± 0.16 to 23.82 ± 3.39 g/L. The characterization of the isolate that presented the highest production yield initially coded as E22 led to the identification of a Klebsiella pneumoniae strain, which, compared with other PHA bacterial producers reported to date, could be considered with high production potential. The strain E22 was grown in 5 different media prepared from fruit peel residues of banana, orange, papaya, watermelon, and melon, to determine its growth and PHA production capabilities in these low-cost media. The results obtained show different bacterial growth yields among the media tested, although PHB production yields and productivities were similar in all these lowcost media. Cellular accumulation of the biopolymer was higher in watermelon peel medium (8.4 × 10−10 g/CFU). These results reveal the potential of K. pneumoniae E22 for PHB production applications and establish encouraging alternatives to be broader explored regarding low-cost media that could enhance the scale-up of bacterial PHA production processes. Keywords Polyhydroxyalkanoates . PHB . Biopolymers . Klebsiella pneumoniae . Fruit residues
1 Introduction * A. M. Rivas-Castillo [email protected] 1
Universidad Tecnológica de la Zona Metropolitana del Valle de México, Blvd. Miguel Hidalgo y Costilla 5, Los Héroes de Tizayuca, 43816 Tizayuca, Hidalgo, Mexico
2
Facultad de Ciencias Químicas, Universidad Juárez del estado de Durango, Av. Veterinaria S/N, Circuito Universitario, Valle del Sur, 34120 Durango, Mexico
3
División de Químico-Biológicas, Universidad Tecnológica de Tecámac, Km 37.5 Carretera Federal México-Pachuca, Colonia Sierra Hermosa, 55740 Tecámac, Estado de México, Mexico
4
Escuela de Ciencias de la Salud, Universidad del Valle de México, Campus Zapopan, Periférico Poniente 7900, Col. Jardines de Collí, 45010 Zapopan, Jalisco, Mexico
5
Grupo de Propiedades Ópticas de la Materia, Centro de Investigaciones en Óptica, A. P
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