Production of recombinant human lysozyme in the milk of transgenic pigs
- PDF / 191,700 Bytes
- 3 Pages / 547.087 x 737.008 pts Page_size
- 19 Downloads / 189 Views
BRIEF COMMUNICATION
Production of recombinant human lysozyme in the milk of transgenic pigs Jia Tong • HengXi Wei • XiaoFang Liu • WenPing Hu • MingJun Bi • YuanYuan Wang QiuYan Li • Ning Li
•
Received: 15 March 2010 / Accepted: 21 May 2010 / Published online: 12 June 2010 Ó Springer Science+Business Media B.V. 2010
Abstract In the swine industry pathogenic infections have a significant negative impact on neonatal survival. Piglets fed with human lysozyme, a natural antibiotic, might be more resistant to gastrointestinal infections. Here we describe the generation of transgenic swine expressing recombinant human lysozyme by somatic cell nuclear transfer. Three cloned female pigs were born, one of which expressed rhLZ at 0.32 ± 0.01 lg/ml in milk, 50-fold higher than that of the pig native lysozyme. Both the transgenic gilts and their progeny appear healthy. Introducing human lysozyme into pigs’ milk has a potential to benefit the piglets by enhancing immune function and defending against pathogenic bacteria, thereby increasing the new born survival rate. This advance could be of great value to commercial swine producers. Keywords Somatic cell nuclear transfer (SCNT) Recombination human lysozyme (rhLZ) Transgenic cloned pig Milk
J. Tong H. Wei X. Liu W. Hu M. Bi Y. Wang N. Li (&) State Key Laboratory for Agrobiotechnology, China Agricultural University, 100193 Beijing, China e-mail: [email protected] Q. Li GenProtein Biotech Ltd., 100193 Beijing, China
Introduction Currently swine producers attempt to maximize both the number of piglets born per litter and piglet survival, but the immune system of the neonate is immature. Weak disease resistance and frequent pathogenic invasion resulting in acute infection (e.g., diarrhoea) can significantly reduce new born survival rate. Antibiotics once were regarded as a universal antimicrobial weapon. However, many bacteria have developed antibiotic-resistant strains, and the number of such resistant species is growing quickly (Wright et al. 2005). Therefore, alternative approaches to treat bacterial infections are urgently needed for healthcare facilities worldwide. Use of enzymes as antimicrobial agents, mirroring defence mechanisms found in nature, has attracted much attention as an antibioticfree approach to treat bacterial infections (Wenk et al. 2000). Recent studies indicate that the consumption of pasteurized hLZ goats’ milk has the potential to improve gastrointestinal health and is protective against an EPEC in young weaned pigs (Brundige et al. 2008). Indeed, recombinant human lysozyme also has been found to be as effective as conventional antibiotics, in promoting growth of poultry (Humphrey et al. 2002) suggesting that it could supplement/replace currently used antibiotic drugs. Most gene transfer experiments in farm animals have been carried out with the purpose of altering growth performance and/or carcass composition (Pursel et al. 1989). Another important and challenging aspect
123
418
Transgenic Res (2011) 20:417–419
of gene transfe
Data Loading...
