Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast
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RESEARCH PAPER
Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast Moumita Bhaumik1 · Gunaseelan Dhanarajan1 · Jayita Chopra1 · RaviRanjan Kumar1 · Chinmay Hazra1 · Ramkrishna Sen1 Received: 14 January 2020 / Accepted: 22 April 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E24: 70–80%), this molecule showed strong emulsion stability over a wide range of pH (2.0–9.0), salinity (0.05%—10%, w/v) and temperature (− 80 °C to + 50 °C). The current study emphasizes on the determination of critical media parameters for improved and stable bioemulsifier production coupled with partial characterization and identification of the molecule. Thus, a proteoglycan-based bioemulsifier with such a stable emulsifying property can serve as a versatile and potential component in food, cosmetics and pharmaceutical formulations. Graphic abstract
Keywords Oleaginous yeast · Meyerozyma caribbica · Stable emulsion · High molecular weight bioemulsifier · Proteoglycan Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00449-020-02361-1) contains supplementary material, which is available to authorized users. * Ramkrishna Sen [email protected]; [email protected] Extended author information available on the last page of the article
Introduction Surface active molecules have drawn attention since decades in various industrial as well as pharmaceutical sectors. Bioemulsifiers and biosurfactants, differing majorly in their
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molecular weight and surface tension reduction property are often termed alternatively due to their pivotal role in environmental application such as bioremediation. Unlike biosurfactants, bioemulsifiers are a set of high molecular weight amphipathic molecule which does not tend to decrease surface tension. These molecules are conferred with properties of micelle formation in oil/water interfaces. The reason for the production of these molecules is mainly attributed towards the uptake of hydrophobic substrates followed by their survival in hydrophobic environment. These surface-active compounds are also well known for its role in cellular physiological processes such as cell signaling and differentiation. It has a characteristic role in biofilm formation which further entails the property of gliding motility as well as enhanced bioavailability of hydrophobic substrates [1]. Surface adherent components play a major role in combating interfacial challenges faced by microorganisms; interfacial mass transfer of substrates serves as an intermediate platform for microbial host interaction [2]. However, many mic
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