Study on Process Optimization for Bioemulsifier Production
A useful bioemulsifier was secreted by Geobacillus sp. XS2 isolated from an oil-contaminated soil sample from Yumen oil field in northern China. The extracellular bioemulsifier was able to form a stable oil/water emulsion. The effects of different bioemul
- PDF / 521,327 Bytes
- 13 Pages / 439.37 x 666.142 pts Page_size
- 23 Downloads / 206 Views
Study on Process Optimization for Bioemulsifier Production Manman Wang, Xingbiao Wang, Chenggang Zheng, Yunkang Chang, Yongli Wang and Zhiyong Huang
Abstract A useful bioemulsifier was secreted by Geobacillus sp. XS2 isolated from an oil-contaminated soil sample from Yumen oil field in northern China. The extracellular bioemulsifier was able to form a stable oil/water emulsion. The effects of different bioemulsifier concentrations on cell growth and activity of fermentation broth with water-soluble and water-insoluble carbon sources were studied. Various carbon sources, nitrogen sources, and C/N ratios were screened, and the culture conditions such as pH and ventilation were optimized through the fermentation progress. After a series of steps of optimization, the maximum bioemulsifier production reached to 21.1 g/L ± 1.15 g/L and more than four times higher than that observed in the initial experiment. And the bacterial strain XS2 showed a good performance to adapt to the environment and strong potential for industrial applications. Keywords Bioemulsifier Geobacillus sp.
Emulsification activity Fermentation optimization
M. Wang College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China X. Wang Y. Chang Z. Huang (&) Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People’s Republic of China e-mail: [email protected] C. Zheng Petroleum Exploration and Production Research Institute, SINOPEC, Beijing 100083, People’s Republic of China Y. Wang (&) Lanzhou Institute of Geology, Chinese Academy of Sciences, Lanzhou 730000, People’s Republic of China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 249, DOI: 10.1007/978-3-642-37916-1_38, Ó Springer-Verlag Berlin Heidelberg 2014
367
368
M. Wang et al.
38.1 Introduction A wide range of biosurfactants with surface activities were produced by various microorganisms [1]. The types of biosurfactants varied very much, including glycolipids, lipopeptides, lipoproteins, phospholipids, fatty acids, lipopolysaccharides, and polysaccharide-protein complexes [2]. Biosurfactants were divided into two classes according to their molecular weight. The high molecular weight biosurfactants, also called bioemulsifiers, were effectively in stabilizing emulsions, instead of efficiently lower surface and interfacial tensions as low molecular weight biosurfactants [3]. As an important member of the family of biosurfactants, many kinds of bioemulsifiers were studied and used in many industries for their significant advantages compared to their chemical counterparts [4, 5]. The characters of bioemulsifiers are as follows: (1) biodegradable, no secondary pollution; (2) nontoxic and harmfulless; (3) digestibly, can be used as food additives; (4) produce biosurfactant
Data Loading...
