Programmed Cell Death Methods and Protocols
This volume contains protocols specifically designed for studying programmed cell death, and also discusses recent advances in techniques that span broader areas of biology that have been recently used or that have potential to be incorporated into cell d
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Hamsa Puthalakath Christine J. Hawkins Editors
Programmed Cell Death Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Programmed Cell Death Methods and Protocols
Edited by
Hamsa Puthalakath and Christine J. Hawkins Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia
Editors Hamsa Puthalakath Department of Biochemistry and Genetics La Trobe Institute for Molecular Science La Trobe University Bundoora, VIC, Australia
Christine J. Hawkins Department of Biochemistry and Genetics La Trobe Institute for Molecular Science La Trobe University Bundoora, VIC, Australia
Videos can also be accessed at http://link.springer.com/book/10.1007/978-1-4939-3581-9 ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3579-6 ISBN 978-1-4939-3581-9 (eBook) DOI 10.1007/978-1-4939-3581-9 Library of Congress Control Number: 2016934448 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: A high resolution image of a Drosophila eye in which the pro-apoptotic gene, hid, was expressed using an eye-specific promoter, resulting in eye ablation. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface The field of programmed cell death is now one of the most dynamic and fast-moving areas of research in biology. Focus of this research field encompasses the basic science of delineating the molecular mechanisms underpinning the cell death process in both mammals and other model organisms (such as C. elegans, Drosophila, and S. cerevisiae) as well as understanding its role in various human pathologies such as cancer, degenerative d
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