Protective effect of Centaurea pallescens Del. against CCl 4 -induced injury on a human hepatoma cell line (Huh7)
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Med Chem Res DOI 10.1007/s00044-013-0563-y
ORIGINAL RESEARCH
Protective effect of Centaurea pallescens Del. against CCl4-induced injury on a human hepatoma cell line (Huh7) Hossam M. Abdallah • Mona A. Mohamed • Allia M. Abdou • Manal M. Hamed • Ashraf B. Abdel-Naim • Osama M. Ashour
Received: 21 October 2012 / Accepted: 27 February 2013 Springer Science+Business Media New York 2013
Abstract Through a hepatoprotective bioassay-guided fractionation of the methanol extract from Centaurea pallescens Del. (Asteraceae), a new acylated flavonoid triglycoside, 40 -methoxy kaempferol 3-O-[a-L-rhamnopyranosyl-(1?3)-(2-O-E-p-coumaroyl)] b-D-glucopyranoside7-O-(4-O-E-p-coumaroyl) a-L-rhamnopyranoside (1) was isolated from the highly active aqueous fraction. In addition, six known compounds were isolated from both aqueous (2– 3) and ethyl acetate soluble fractions (4–7). The structure of (1) was determined by comprehensive analysis of 1D and 2D NMR and mass spectral data. The protective effects of the methanol extract of C. pallescens and its fractions on the human hepatoma cell line were evaluated using Silymarin as a positive control. Hepatoprotection was assessed through determination of aspartate aminotransferase (AST), alanine transaminase (ALT), and superoxide dismutase (SOD)
activities in addition to glutathione (GSH) levels before and after incubating the cells with carbon tetrachloride. Compound 1, the major constituent of the aqueous fraction, showed a significant cytoprotection at 100 lg/mL as evidenced by decreasing ALT activity to 18.6 ± 0.12, and enhancing SOD activity to 264.6 ± 4.3 U/mL. Meanwhile, compound 2 at 10 lg/mL decreased AST activity to 5.8 ± 2.4 U/mL. Moreover, Compounds 2 and 3 at 1,000 lg/mL significantly enhanced GSH levels. In conclusion, the protective effects of C. pallescens extract, its fractions and compounds 1–3 are concluded to be partly mediated by its antioxidant activity. Keywords Centaurea pallescens Acylated methoxy kaempferol Hepatoprotective
Introduction Electronic supplementary material The online version of this article (doi:10.1007/s00044-013-0563-y) contains supplementary material, which is available to authorized users. H. M. Abdallah Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia H. M. Abdallah Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt M. A. Mohamed (&) A. M. Abdou M. M. Hamed Department of Medicinal Chemistry, Theodor Bilharz Research Institute (TBRI), Giza 12411, Egypt e-mail: [email protected] A. B. Abdel-Naim O. M. Ashour Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
The liver is one of the most important organs in the body, playing a pivotal role in regulating various physiological processes. It is involved in several vital functions, such as metabolism, secretion, and storage in addition to its vast capacity to detoxify noxious substances. Hepatic injury inflicted by hepat
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