Protein expression in accessions of Chinese onion with different allelopathic potentials under monocropping and intercro
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ORIGINAL PAPER
Protein expression in accessions of Chinese onion with different allelopathic potentials under monocropping and intercropping systems Yang Yang • Fengzhi Wu • Xingang Zhou
Received: 16 August 2012 / Revised: 6 March 2013 / Accepted: 7 March 2013 / Published online: 30 March 2013 Ó Franciszek Go´rski Institute of Plant Physiology, Polish Academy of Sciences, Krako´w 2013
Abstract Protein expressions of Chinese onion accessions grown under monoculture and intercropped with cucumber were evaluated in pot experiments. Chinese onion accessions used were L04 (with weak allelopathic potential) and L06 (with strong allelopathic potential). Root proteins were separated by two-dimensional electrophoresis and the variable expressed protein spots were identified with MALDI-TOFTOF mass spectrometer. Forty-seven identified proteins were classified into nine functional categories. Compared monocropping and intercropping, 31 identified variable protein spots were classified into energy metabolism (14 %), phenylpropanoid biosynthesis (28 %), organosulfur compounds biosynthesis (25 %), carbohydrate metabolism (10 %), fatty acid hydrogen peroxide metabolism (9 %), protein translation (3 %), other function (3 %), and no assigned function (9 %). Compared Chinese onion accessions of differing allelopathy potentials, 22 identified variable protein spots were classified into energy metabolism (18 %), phenylpropanoid biosynthesis (27 %), organosulfur compounds biosynthesis (23 %), carbohydrate metabolism (9 %), nucleosome component (4 %), other function (14 %), and no assigned function (5 %). The level of variable-expressed proteins involved in phenylpropanoid and organosulfur
Communicated by M. J. Reigosa. Y. Yang F. Wu (&) X. Zhou Department of Horticulture, Northeast Agricultural University, Mucai 59, Xiangfang, Harbin 150030, People’s Republic of China e-mail: [email protected] Y. Yang Department of Biological Science and Technology, Wuhan Bioengineering Institute, Hanshi 1, Yangluo Economic Development Zone, Wuhan 430415, People’s Republic of China
compounds biosynthesis significantly upregulated in treatments intercropped with cucumber. These results suggested that putative allelochemicals of Chinese onion were mainly produced by phenylpropanoid biosynthesis and organosulfur compounds biosynthesis pathway. Keywords Protein Monocropping Intercropping Allelopathic potential Chinese onion
Introduction Allelopathy is a phenomenon involving either direct or indirect and either beneficial or adverse effects of a plant (including microorganisms) on another plant through the release of chemicals in the environment (Zhao et al. 2010). It plays an important role in the formation and succession of natural plant communities, in crop resistance to disease and weed control, in continuous cropping, intercropping and other cultivation systems (Jose and Gillespie 1998; Rice 1995). Intercropping has been reported to enhance yield and yield stability (Willey 1979), increase resourceuse efficiency, especially for nitro
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