Proteome sequencing and analysis of Ophiocordyceps sinensis at different culture periods
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RESEARCH ARTICLE
Open Access
Proteome sequencing and analysis of Ophiocordyceps sinensis at different culture periods Bo Zhang1, Bo Li1, Xiao-Hui Men1, Zhe-Wen Xu1, Hui Wu2,3, Xiang-Tian Qin2,3, Feng Xu2,3, Yi Teng2,3, Shui-Jin Yuan2,3, Li-Qun Jin1, Zhi-Qiang Liu1* and Yu-Guo Zheng1
Abstract Background: Ophiocordyceps sinensis is an important traditional Chinese medicine for its comprehensive active ingredients, such as cordycepin, cordycepic acid, and Cordyceps polysaccharide. O. sinensis zjut, a special strain isolated from O. sinensis, has similar pharmacological functions to wild O. sinensis. Currently, O. sinensis with artificial cultivation has been widely studied, but systematic fundamental research at protein levels has not been determined. Results: Proteomes of O. sinensis zjut at different culture periods (growth period, 3rd day; pre-stable period, 6th day; and stable period, 9th day) were relatively quantified by relative isotope markers and absolute quantitative technology. In total, 4005 proteins were obtained and further annotated with Gene Ontology, Kyoto Encyclopedia of Genes and Genomes database. Based on the result of the annotations, metabolic pathways of active ingredients, amino acids and fatty acid were constructed, and the related enzymes were exhibited. Subsequently, comparative proteomics of O. sinensis zjut identified the differentially expressed proteins (DEPs) by growth in different culture periods, to find the important proteins involved in metabolic pathways of active ingredients. 605 DEPs between 6dVS-3d, 1188 DEPs between 9d-VS-3d, and 428 DEPs between 9d-VS-6d were obtained, respectively. Conclusion: This work provided scientific basis to study protein profile and comparison of protein expression levels of O. sinensis zjut, and it will be helpful for metabolic engineering works to active ingredients for exploration, application and improvement of this fungus. Keywords: O. sinensis zjut, Proteome, Differentially expressed proteins, Active ingredients
Background Ophiocordyceps sinensis is an important traditional Chinese medicine and healthy food in China [1–3]. Previous studies have revealed that active ingredients of O. sinensis, including D-mannitol, cordycepin, purine nucleotides and polysaccharide, have various pharmacological functions, such as adaptogenic activity, * Correspondence: [email protected] 1 Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China Full list of author information is available at the end of the article
immunomodulatory effects, antioxidant activity and anti-cancer [4, 5]. However, due to high demand and insufficient supply for wild O. sinensis, the medicinal value development has been seriously limited [6, 7]. Recently, several strains that isolated from wild O. sinensis have similar pharmacological functions to wild O. sinensis [8], representing an useful alternative for production. Moreover, new potential drugs and active compounds deriv
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