Proteomics in Functional Genomics Protein Structure Analysis
A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications
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Proteomics in Functional Genomics Protein Structure Analysis Edited by P. Jolles and H. Jornvall
Springer Basel AG
Editors Prof. Dr. P. Jolles Laboratoire de Chimie des Substances Naturelles URA C.N.R.S. No. 401 Museum National d'Histoire Naturelle 63, rue Buffon F-75005 Paris France
Prof. Dr. H. Jomvall Department of Medical Biochemistry and Biophysics Karolinska Institutet SE-l71 77 Stockholm Sweden
Library of Congress Cataloging-in-Publication Data Proteomics in functional genomics : protein structure analysis / edited by P. Jolles, H. Jomvall. p. ; cm. - (EXS ; 88) Inc1udes bibliographical references and index. ISBN 3764358858 (hard cover : alk. paper) 1. Proteins - Structure. 2. Proteins - Analysis. 1. Title: Protein structure analysis. II. Jolles, Pierre, 1927-III. Jomvall, Hans. Iv. Series. [DNLM: 1. Proteins - chemistry. 2. Genome. 3. Protein Structure. 4. Sequence Analysis. QU 55 P9825 2000] QP551.P7567 2000 572'.633-dc21 99-059890 Deutsche Bibliothek Cataloging-in-Publication Data Proteomics in functional genomics : protein structure analysis / ed. by P. Jolles ; H. Jomvall. Basel ; Boston; Berlin: Birkhliuser, 2000 (EXS; 88) ISBN 978-3-0348-9576-7 ISBN 978-3-0348-8458-7 (eBook) DOI 10.1007/978-3-0348-8458-7
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© 2000 Springer Basel AG Originally published by Birkhăuser Verlag, Basel, Switzerland in 2000 Cover illustration: identification ofa spot from a 2D-gel ana1ysis (page 12)
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Contents List of Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . VII Hans Jornvall and Pierre Jolles Protein structure analysis of today: Proteomics in functional genomics.
XI
Ettore Appella, David Arnott, Kazuyasu Sakaguchi and Peter J. Wirth Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..
1
Kris Gevaert, Tony Houthaeve and Joel Vandekerckhove Techniques for sample preparation including methods for concentrating peptide samples . . . . . . . . . . . . . . .
29
UlfHellman Sample preparation by SDS/PAGE and in-gel digestion
43
Julio E. Celis and Pavel Gromov High-resolution two-dimensional gel electrophoresis and protein identification using w
