Functional Proteomics Methods and Protocols
This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especial
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Xing Wang Matthew Kuruc Editors
Functional Proteomics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Functional Proteomics Methods and Protocols
Edited by
Xing Wang Array Bridge Inc., St. Louis, MO, USA
Matthew Kuruc Biotech Support Group LLC, Monmouth Junction, NJ, USA
Editors Xing Wang Array Bridge Inc. St. Louis, MO, USA
Matthew Kuruc Biotech Support Group LLC Monmouth Junction, NJ, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8813-6 ISBN 978-1-4939-8814-3 (eBook) https://doi.org/10.1007/978-1-4939-8814-3 Library of Congress Control Number: 2018957271 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Over the past two decades, tremendous progress has been made in the field of proteomics, the purpose of which is to find systemic differences in protein populations. Once established, measurable protein markers can then help to define biological mechanism, disease, identify therapeutic targets, and offer better precision for personalized medical interventions. Proteomics, like other “omics” analyses, is data driven and can generate unbiased protein profiles for a variety of end points that can contrast, for example, treated vs. untreated cell models or healthy vs. diseased tissue; it has provided us with more in-depth understanding of many biological systems and diseases. This progress in the field of proteomics parallels with th
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