A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system
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RESEARCH PAPER
A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system Wenguang Zhang 1,2 & Shengjun Bu 2 & Huasong Bai 2 & Chengyou Ma 3 & Li Ma 2 & Hongguo Wei 2 & Xiu Liu 2 & Zehong Li 1 & Jiayu Wan 2 Received: 30 June 2020 / Revised: 4 August 2020 / Accepted: 28 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to Salmonella typhimurium (S. typhimurium), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of S. typhimurium from 102 to 108 CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria. Keywords Salmonella typhimurium . Aldehyde dehydrogenase . Nanocomposite . pH meter
Introduction Point-of-care (POC) diagnostic technology with high performance is becoming increasingly popular, playing a vital role in maintaining a healthy society and food safety [1, 2]. Qualitative or quantitative detection of clinically relevant biological targets, such as small molecules, proteins, nucleic acids, and pathogens, is of crucial significance for improving the level of diagnosis, timely and effective treatment of diseases, and prevention of pathogen infection [3]. Among these approaches, biosensors with the functions of target-specific Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02928-7) contains supplementary material, which is available to authorized users. * Zehong Li [email protected] * Jiayu Wan [email protected] 1
College of Life Science, Jilin Agricultural University, Changchun 130118, Jilin, China
2
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, Jilin, China
3
College of Geo-Exploration Science and Technology, Jilin University, Changchun 130026, Jilin, China
recognition and signal transduction play an important prominent role [4]. Biosensors convert biorecognition element of binding to its target into physicochemical detection signals [5]. To facilitate the use of biosensors at POC locations, several physiochemical detectable signals, including glucose [6], pressure [7], temperature [8], hydrogen [9], and electrochemical signal [10], are used as readouts. These signal transduction strategies revamp the commercially available hand-held meters, such as personal glucose meter (PGM), pressure meter, thermometer, hydrogen detector, and screen-printed carbon electrodes [11]. Smartphone has also been used
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