Centipeda minima ( Ebushicao ) extract inhibits PI3K-Akt-mTOR signaling in nasopharyngeal carcinoma CNE-1 cells
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RESEARCH
Centipeda minima (Ebushicao) extract inhibits PI3K‑Akt‑mTOR signaling in nasopharyngeal carcinoma CNE‑1 cells Yu‑qing Guo1,2, Hai‑yan Sun1,2, Chi‑on Chan2, Bei‑bei Liu2, Jian‑hong Wu2, Shun‑wan Chan2, Daniel Kam‑Wah Mok2, Anfernee Kai‑Wing Tse3, Zhi‑ling Yu3 and Si‑bao Chen1,2*
Abstract Background: Centipeda minima (Ebushicao) has been used for the treatment of various diseases, such as nasal allergies, rhinitis and sinusitis, nasopharyngeal carcinoma, cough, and headache. This study aims to investigate the anticancer activities of Centipeda minima ethanol extracts (CME) against nasopharyngeal carcinoma cell CNE-1 and their underlying mechanism. Methods: CNE-1 cells were treated with different concentrations (15–50 μg/mL) of CME for different time intervals (24, 48, and 72 h). Cytotoxicity of CME was determined by MTT assay. Cell morphological changes were observed by fluorescence microscopy after HO 33258 staining. Cell cycle status was evaluated by flow cytometry following pro‑ pidium iodide staining. Apoptosis was detected by flow cytometry following annexin V-FITC/PI staining. The levels of apoptosis-associated and PI3K-Akt-mTOR signaling related proteins were measured by western blotting analysis. Results: CME (15–50 μg/mL) significantly inhibited the proliferation of CNE-1 in a dose- and time-dependent manner (P = 0.026 for 15 μg/mL, P
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