Characterization of the glycans involved in sea urchin Paracentrotus lividus reversible adhesion
- PDF / 3,457,023 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 84 Downloads / 153 Views
ORIGINAL PAPER
Characterization of the glycans involved in sea urchin Paracentrotus lividus reversible adhesion Mariana Simão1 · Mariana Moço1 · Luís Marques2,3 · Romana Santos1 Received: 1 April 2020 / Accepted: 14 May 2020 © The Author(s) 2020
Abstract Sea urchins have hundreds of specialized adhesive organs, the tube feet, which play a key role in locomotion, substrate attachment and food capture. Tube feet are composed by two functional units: a proximal cylindrical stem that is mobile and flexible, attached to a distal flattened disc that produces adhesive secretions. Oral tube feet discs possess a specialized duo-glandular epidermis that produces adhesive and de-adhesive secretions, enabling strong but reversible adhesion to the substrate. Due to the growing interest in biomimetic adhesives, several studies have been carried out to characterize sea urchin adhesives, and up to date, it has been shown that it is composed by proteins and glycans. The protein fraction has been the subject of several studies, that pin-pointed several adhesion-related candidates. Contrastingly, little is known about the glycans that compose sea urchin adhesives. This study aims at contributing to this topic by focusing on the characterization of the glycosidic fraction of the adhesive secreted by the sea urchin Paracentrotus lividus (Lamarck, 1816), using a battery of 22 lectins, applied to 3 complementary techniques. Our results show that five lectins label exclusively the disc adhesive epidermis and simultaneously the secreted adhesive, being, therefore, most likely relevant for sea urchin adhesion. In addition, it was possible to determine that the glycosidic fraction of the adhesive is composed by a high molecular weight glycoprotein containing N-acetylglucosamine oligomers.
Introduction
Responsible Editor: M. Byrne. Reviewed by undisclosed experts. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00227-020-03707-9) contains supplementary material, which is available to authorized users. * Romana Santos [email protected] 1
Centro de Ciências do Mar e do Ambiente (MARE), Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749‑016 Lisboa, Portugal
2
Centro de Ecologia, Evolução e Alterações Ambientais (cE3c), Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749‑016 Lisboa, Portugal
3
Instituto de Biossistemas e Ciências Integrativas (BioISI), Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749‑016 Lisboa, Portugal
Like other echinoderms, sea urchins have an ambulacral system made up of hundreds of specialized adhesive organs, called tube feet (Fig. 1a), used for substrate attachment, locomotion and food capture (Flammang et al. 2016). Tube feet are composed by a stem and a disc (Fig. 1b). The stem is attached to the animal’s test, being flexible and mobile. The disc is at the distal end of the stem, has a flat shape and viscoelastic properties ideal for contact and ad
Data Loading...
