Clinical endodontic procedures modulate periapical cytokine and chemokine gene expressions
- PDF / 605,354 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 2 Downloads / 193 Views
ORIGINAL ARTICLE
Clinical endodontic procedures modulate periapical cytokine and chemokine gene expressions Lucas Moreira Maia 1 & Marcela Carvalho Espaladori 1 & Julia Maria Braga Diniz 1 & Warley Luciano Faria Tavares 1 & Luciana Carla Neves de Brito 2 & Leda Quércia Vieira 3 & Antônio Paulino Ribeiro Sobrinho 1 Received: 16 October 2019 / Accepted: 16 February 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Objectives To identify the gene expression of the cytokines IL-9, TNF-α, IL-1, INF-γ, IL-17A, and IL-10 and the chemokines CCL-2/MCP-1 and CCR-6 in the periapical fluid of human root canal infections. Materials and methods Twenty samples were collected immediately and 7 days after the cleaning and shaping procedures (after reducing the intracanal microbial load) in an attempt to characterize the expression of these genes. The endogenous expression levels of cytokines and chemokines were analyzed by real-time polymerase chain reaction. The Shapiro-Wilk and the Wilcoxon tests analyzed data. Results Significantly higher levels of the IL-9, INF-γ, TNF-α, IL-1, and IL-10 markers on day 7 were observed compared with day 0 (p < 0.05). However, IL-17A and the chemokines CCL-2/MCP-1 and CCR-6 did not show a significant difference in mRNA expression when comparing both timepoints (p > 0.05). Conclusions The clinical variation of the periapical immune status after endodontic therapy suggests that the cytokine and chemokine-mediated pro-inflammatory response appears to be modulated in an IL-10/IL-9-dependent manner. Clinical relevance Few studies have investigated the role of Th9 cells in periapical lesions. IL-9 presents exciting plasticity, performing immunosuppressive actions, and it is also capable of changing their phenotype in the presence of IL-17. Hence, it is relevant to investigate its role in the context of the known mediators involved the periapical immune process. Keywords Interleukin . IL-9 . TH9 . Apical periodontitis . Cytokine and chemokine
Introduction The pulp-dentin complex is composed of the pulp organ and the surrounding dentin walls that form a physical barrier against opportunistic pathogens [1, 2]. Normally, resident microbiota are not present in the root canal; however, when the pulp vascular supply is collapsed, the root canal becomes a fertile environment for opportunistic pathogens to grow [3]. Such infections lead to periradicular bone resorption and migration of several inflammatory cells to this site, subsequently * Antônio Paulino Ribeiro Sobrinho [email protected] 1
Department of Operative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
2
Faculty of Dentistry, University of Itaúna, Itaúna, MG, Brazil
3
Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
promoting immunological surveillance against microbial pathogens [1, 4]. It was previously proposed that protective and destructive immunoreactions take place simul
Data Loading...
