Colorimetric determination of ascorbic acid using a polyallylamine-stabilized IrO 2 /graphene oxide nanozyme as a peroxi
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ORIGINAL PAPER
Colorimetric determination of ascorbic acid using a polyallylamine-stabilized IrO2/graphene oxide nanozyme as a peroxidase mimic Huiyuan Sun 1,2 & Xueliang Liu 1 & Xinhuan Wang 1 & Qiusen Han 1,3 & Cui Qi 1 & Yanmei Li 2 & Chen Wang 1,3 & Yongxiang Chen 2 & Rong Yang 1,3 Received: 13 May 2019 / Accepted: 6 October 2019 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The authors describe a peroxidase-mimicking nanozyme composed of IrO2 and graphene oxide (GO). It was synthesized from monodisperse IrO2 nanoparticles with an average diameter of 1.7 ± 0.3 nm that were prepared by pulsed laser ablation in ethanol. The nanoparticles were then placed on polyallylamine-modified GO nanosheets through electrostatic interaction. The peroxidase-like activity of the resulting nanocomposites was evaluated by catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2. Kinetic results demonstrated that the catalytic behavior of the nanocomposites follows MichaelisMenten kinetics. Experiments performed with terephthalic acid and cytochrome C confirmed that the peroxidase-like activity originated from the electron transfer mechanism rather than from generation of hydroxy radicals. The peroxidase-like activity is inhibited in the presence of ascorbic acid (AA). Based on this property, a colorimetric assay was developed for the determination of AA by exploiting the peroxidase-like activity of IrO2/GO nanocomposites. The linear relationship between absorbance at 652 nm and the concentration of AA was acquired. The limit of detection for AA is 324 nM. Further applications of the method for AA detection in real samples were also successfully demonstrated. Keywords Pulsed laser ablation . IrO2/GO nanocomposites . Nanozymes . Nanoprobes . Peroxidase-like activity . Colorimetric detection
Introduction Huiyuan Sun and Xueliang Liu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-019-3897-4) contains supplementary material, which is available to authorized users. * Yongxiang Chen [email protected] * Rong Yang [email protected] 1
CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS center for Excellence in Nanoscience, National Center for Nanoscience and Technology, UCAS, Beijing 100190, People’s Republic of China
2
Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, People’s Republic of China
3
Sino-Danish College, UCAS, Sino-Danish Center for Education and Research, Beijing 100190, People’s Republic of China
Ascorbic acid (AA) participates in many physiological and biochemical processes as a cofactor for several enzymes [1, 2]. Insufficient or excessive AA can lead to scurvy and anemia, accompanied by some psychological abnormalities [3]. Therefore, it is important to develop a convenient and rapid me
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