Comparing the nasal bacterial microbiome diversity of allergic rhinitis, chronic rhinosinusitis and control subjects
- PDF / 742,365 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 63 Downloads / 195 Views
RHINOLOGY
Comparing the nasal bacterial microbiome diversity of allergic rhinitis, chronic rhinosinusitis and control subjects Weigang Gan1 · Fengjuan Yang2 · Juan Meng1 · Feng Liu1 · Shixi Liu1 · Junming Xian1 Received: 15 May 2020 / Accepted: 19 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Purpose Until now, the microbiome of the nasal cavity and its contribution to nasal mucosal disease has remained poorly understood. The advent of cultivation-free molecular methods makes it possible to characterize the total microbiome of the nasal cavity. We sought to assess the microbial diversity and composition of the middle meatus in allergic rhinitis (AR) patients, chronic rhinosinusitis patients without polyps (CRSsNP) and a control population to determine the microbiota associated with the pathogenesis of AR and CRSsNP. Methods Microbial characterization was determined using 16S rRNA gene sequencing of 122 nasal swabs collected from patients with AR (n = 52) and CRSsNP (n = 37), and from healthy controls (n = 33). Results There was no difference in nasal microbiome richness and diversity among the three groups, and the dominant phyla were similar among three groups including Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. However, Spirochaetae abundance was significantly higher in AR than in the control group after FDR correction (FDR p = 0.021). At the genus level, although there was no statistical significance after FDR correction, there was a trend that Pseudomonas and Peptostreptococcaceae abundance were higher in AR than in controls (p = 0.005, p = 0.005) and CRSsNP (p = 0.023, p = 0.034); Lactobacillus abundance was lower in AR than in controls (p = 0.021); Moraxella abundance was lower in CRSsNP than in controls (p = 0.006); Haemophilus abundance was higher in CRSsNP than in AR (p = 0.003) but lower in AR than in controls (p = 0.018). Conclusion These results suggested that microbial dysbiosis may play a role in the pathogenesis of heterogeneous nasal mucosal inflammation. Keywords 16S rRNA · Allergic rhinitis · Bacteria · Chronic rhinosinusitis · Microbiota
Introduction
Weigang Gan and Fengjuan Yang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00405-020-06311-1) contains supplementary material, which is available to authorized users. * Juan Meng [email protected] 1
Department of Otorhinolaryngology‑Head and Neck Surgery, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, Sichuan, People’s Republic of China
Department of Otorhinolaryngology, Nanchong Fifth People’s Hospital, Nanchong, Sichuan, People’s Republic of China
2
The prevalence of inflammatory disease of the nasal mucosa, including allergic rhinitis (AR) and chronic rhinosinusitis (CRS), is increasing annually all around the world. Despite great progress has been made in the pathogenic mechanism of these diseases, the exact etiology remains unknown. With recent advanc
Data Loading...
