Comparison of the detection performance of two different one-step-combined test strips with fluorescent microspheres or
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RESEARCH
Open Access
Comparison of the detection performance of two different one-step-combined test strips with fluorescent microspheres or colored microspheres as tracers for influenza A and B viruses Qingjun Pan1† , Weiquan Wu2†, Shuzhen Liao1†, Sijie Wang2, Chunfei Zhao1, Chen Li3* and Ping Wu2*
Abstract Background: Influenza A and B viruses mainly cause respiratory infectious disease. Till now, few tests are able to simultaneously detect both, especially in primary medical establishments. Methods: This study was designed to compare the performance of two different one-step-combined test strips for the detection of influenza A and B: one strip with fluorescent microspheres for tracers (FMT); and the other strip with colored microspheres for tracers (CMT). To test the strips, cultures of influenza A, B, and other pathogenic viruses were used, in addition to 1085 clinical specimens from symptomatic patients with respiratory infections. Real-time RT-PCR was also considered as a reference method used to detect the different results of FMT and CTM. Results: Detection thresholds for influenza A and B cultures using serial dilutions revealed that the sensitivity of FMT was higher than that of CMT (both P < 0.05). With the culture mixtures of Coxsackie virus (A16), enteric cytopathic human orphan virus (ECHO type30), enterovirus (EV71), rotavirus (LLR strain), and enteric adenovirus (AdV 41), specificity assessment demonstrated that there was no cross reaction during the usage of the two test strips as shown by the results which were negative. In the detection of influenza A in 1085 clinical specimens, the total coincidence rate was 96.7%, the positive coincidence rate was 97.1%, and the negative coincidence rate was 96.7%. In the case of influenza B detection, the total coincidence rate was 99.1%, the positive coincidence rate was 92.6%, and the negative coincidence rate was 98.5%. In addition, with influenza A or B real-time RT-PCR detection method, the results showed that, for influenza A, 26 of the 33 specimens that negative with CMT but positive with FMT, showed positive results, and none of the 3 specimens that positive with CMT but negative with FMT showed a positive result; For influenza B, 12 of the 15 specimens that negative with CMT but positive with FMT, showed positive results, and none of the 5 specimens that positive with CMT but negative with FMT showed a positive result. Conclusions: FMT performed better than CMT in the combined detection of influenza A and B viruses. Keywords: Influenza A, Influenza B, Fluorescent microspheres, Colored microspheres, One step combined test
* Correspondence: [email protected]; [email protected] † Qingjun Pan, Weiquan Wu and Shuzhen Liao contributed equally to this work. 3 Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China 2 Clinical Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China Full list of author information is available at the end of the article © The
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